Morphological evaluation of chondrogenic potency in passaged cell populations(CELL AND TISSUE ENGINEERING)

概要

論文の詳細を見る
The present study describes the morphological assessment of chondrogenic potency during a cell expanding process through serial subculturing of rabbit chondrocytes at different levels of population doublings (PD) in a T-flask with a conventional polystyrene surface. The passaged populations were seeded on a high-density collagen surface (CL surface) and in a collagen gel (CL gel) scaffold to evaluate the planar and spatial morphologies of the chondrocytes, respectively, as well as the gene expressions of mRNA for collagen types I and II. The planar morphological estimation was based on roundness (R_c) of chondrocyte cells at different PD values after 1 day incubation on the CL surface. The frequency of round-shaped cells with R_c>0.9 (f_R) decreased with increasing PD values, accompanied by an increase in collagen type I mRNA level. At PD=17.8, the frequency reached f_R=0.12, which was less than one-sixth of that at PD=0. A similar trend was found with respect to the passaged chondrocytes embedded in the CL gels by estimating the spatial morphology in terms of sphericity (S_c) determined 4 days after seeding. With an increase in PD value, the frequency in spherical-shaped cells with S_c>0.9 (f_s) decreased and the mRNA expression of collagen type I increased, giving f_s=0.28 at PD=17.8 which was less than a quarter of that at PD=0. From these results, the cell morphologies on the CL surface and in the CL gel were proposed as indicators for understanding chondrogenic potentials concerning the phenotypes and differentiated states in the population during cell expansion, ultimately leading to quality control of tissue-engineered cartilage.
社団法人日本生物工学会の論文

Morphological evaluation of chondrogenic potency in passaged cell populations(CELL AND TISSUE ENGINEERING)

スポンサーリンク

概要

著者

関連論文

スポンサーリンク