Production and Release of Anthraquinone Pigments by Hairy Roots of Madder (Rubia tinctorum L.) under Improved Culture Conditions

概要

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Carbon and nitrogen sources in the medium were selected from the culture of madder hairy roots producing anthraquinone pigments. The growth and pigment formation of the hairy roots were significantly enhanced by using modified Murashige-Skoog (MS) medium containing fructose and nitrate as the sole carbon and nitrogen sources, respectively, compared with those obtained in conventional MS medium with sucrose. Repeated-batch culture of the hairy roots was carried out, with pigment release into the medium obtained by means of O_2 starvation treatment. In three pigment-release operations during 29-d culture, the total amount of released pigments was 21mg/dm^3,representing an average production rate to 0.72mg/(dm^3・d).
社団法人日本生物工学会の論文
1994-01-25