3-Keto Reductase Activity of Estradiol-17β Dehydrogenase and Its Contribution to Androgen Metabolism
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概要
- 論文の詳細を見る
Hepatic estradiol-17β dehydrogenase from chickens catalyzed the reduction of the 3-keto group of androgens such as 5α-dihydrotestosterone and 5α-androstane-3,17-dione as well as the 17-ketoreduction of 4-androstene-3,17-dione and dehydroepiandrosterone. The reaction products from 17-ketosteroid and 3-ketosteroid substrates were identified as 17β-hydroxysteroids and 3β-hydroxysteroids, respectively, by thin layer chromatography, high performance liquid chromatography and gas chromatography. Barbital inhibited both 17β-estradiol dehydrogenase and 5α-androstane-3,17-dione reductase activity noncompetitively giving the same kinetic constant, K_i=50 μM. 5α-Androstane-3,17-dione competitively inhibited 17β-estradiol dehydrogenase activity. These results indicate that chicken liver estradiol-17β dehydrogenase is in fact a 3β-and 17β-hydroxysteroid dehydrogenase and that both 3- and 17-ketosteroids bind to the same catalytic site.
- 公益社団法人日本薬学会の論文
著者
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TERADA Tomoyuki
Laboratory of Biochemistry, Faculty of Pharmacy, Osaka Ohtani University
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NISHIHARA Tsutomu
Laboratory of Environmental Biochemistry, Graduate School of Pharmaceutical Sciences Osaka Universit
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Mizoguchi Tadashi
Laboratory Of Biochemistry Faculty Of Pharmaceutical Sciences Osaka University
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Mizoguchi T
Faculty Of Science Gakushuin University
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Terada Tomoyuki
Laboratory Of Biochemistry Faculty Of Pharmaceutical Sciences Osaka University
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Terada Tomoyuki
Laboratory Of Biochemistry And Molecular Biology Graduate School Of Pharmaceutical Sciences Osaka Un
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Nishinaka T
Osaka Univ. Pharmaceutical Sci. Osaka Jpn
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Mizoguchi Tadashi
Laboratory Of Bio-functional Engineering Faculty Of Pharmaceutical Sciences Osaka University
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NISHINAKA Tohru
Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Osaka University
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KINOSHITA Yumiko
Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Osaka University
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TERADA Naomi
Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Osaka University
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Nishihara Tsutomu
Laboratory Of Environmental Biochemistry Graduate School Of Pharmaceutical Sciences Osaka University
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Nishinaka Tohru
Laboratory Of Bio-functional Engineering Faculty Of Pharmaceutical Sciences Osaka University
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Terada Naokata
Laboratory Of Biochemistry Faculty Of Pharmaceutical Sciences Osaka University
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Terada Tomoyuki
Laboratory Of Biochemistry And Molecular Biology Graduate School Of Pharmaceutical Sciences Osaka Un
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Kinoshita Y
Laboratory Of Biochemistry Faculty Of Pharmaceutical Sciences Osaka University
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Mizoguchi Tadaaki
Research Center Of Supercritical Fluid Technology/research Institute For Environmental Conservation
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Mizoguchi Tadashi
Faculty Of Pharmaceutical Sciences Osaka University
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