Identification of Zinc Finger Proteins Bound to a Silencer Region in the Rat Glutathione Transferase P Gene
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概要
- 論文の詳細を見る
The rat glutathione transferase P (GST-P) gene is strongly induced during chemical hepatocarcinogenesis, whereas mRNA of this gene is rarely expressed in normal rat liver. We previously identified a silencer region in the promoter of this gene. This silencer has several DNA binding sites and at least three proteins (Silencer factor-A, -B, and -C (SF-A, SF-B, and SF-C)) bind to these sites. We previously cloned and characterized the Nuclear Factor 1 (NF1) family and the CCAAT/enhancer-binding protein (C/EBP) family as SF-A and SF-B, respectively. However, SF-C which binds to GST-P silencer 2 (GPS2) remains to be cloned. By screening using yeast one-hybrid system, several zinc finger proteins were identified as a candidate of SF-C. The gel-mobility shift analyses showed that BTEB2, EZF, LKLF, TFIIIA, TIEG1, and novel zinc finger protein MZFP bound to GPS2 with different affinities. Several proteins of these are known to be transcriptional activators or repressors, suggesting that zinc finger proteins bind to GPS2 and regulate GST-P expression in the rat liver.
- 公益社団法人日本薬学会の論文
- 2001-02-01
著者
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OSADA Shigehiro
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University
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NISHIHARA Tsutomu
Laboratory of Environmental Biochemistry, Graduate School of Pharmaceutical Sciences Osaka Universit
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Osada Shigehiro
Laboratory of Environmental Biochemistry, Graduate School of Pharmaceutical Sciences, Osaka Universi
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Imagawa Masayoshi
Laboratory of Environmental Biochemistry, Graduate School of Pharmaceutical Sciences, Osaka Universi
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Nishinaka T
Osaka Univ. Pharmaceutical Sci. Osaka Jpn
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Oshima K
Laboratory Of Environmental Biochemistry Graduate School Of Pharmaceutical Sciences Osaka University
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Nishihara T
Department Of Oral Microbiology Kyushu Dental College
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Osada Shigehiro
Department Of Molecular Biology Graduate School Of Pharmaceutical Sciences Nagoya City University
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Nishihara Tsutomu
Laboratory Of Environmental Biochemistry Graduate School Of Pharmaceutical Sciences Osaka University
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TANABE Atsuhiro
Laboratory of Environmental Biochemistry Graduate School of Pharmaceutical Sciences, Osaka Universit
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OSHIMA Kazumi
Laboratory of Environmental Biochemistry Graduate School of Pharmaceutical Sciences, Osaka Universit
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Tanabe Atsuhiro
Laboratory Of Environmental Biochemistry Graduate School Of Pharmaceutical Sciences Osaka University
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Nishihara Tsutomu
Laboratory Of Environmental Biochemistry Graduate School Of Pharmaceutical Sciences Osaka University
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Nishihara T
Fac. Of Pharmaceutical Sciences Hyogo Univ. Of Health Sciences
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Nishihata Tomohiro
Institute Of Natural Medicine University Of Toyama
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