Histone Methyltransferase PR-Set7 and Histone Variant H2A.Z, Induced during Hepatocarcinogenesis, Repress the Promoter Activity of the Tumor Marker Gene and the Ras-Induced Colony Formation Activity
スポンサーリンク
概要
- 論文の詳細を見る
Genetic and epigenetic studies are required to understand molecular mechanisms of carcinogenesis and tumorigenesis. Although alterations of DNA methylation and histone modification profiles are observed in cancer cells, the knowledge of the epigenetic regulatory factors involved in carcinogenesis is insufficient. In this study, we showed that the histone variant, H2a.z, and the histone methyltransferase, Pr-set7/Set8/KMT5a, were up-regulated during chemically induced hepatocarcinogenesis. During this process, the glutathione S-transferase placental form (GST-P), which is completely repressed in normal liver, is strongly induced and is therefore an excellent tumor marker. Reporter analysis performed using the regulatory region of the Gst-p gene revealed that H2A.Z and PR-SET7 repressed Gst-p promoter activity. The enhancer element responsible for hepatocarcinogenic-specific gene expression was required for repression by H2A.Z, and the negative regulation by PR-SET7 mediated the regulatory element but not the enhancer. Furthermore, we examined the effects of overexpression of H2A.Z and PR-SET7 on the colony formation activity of mouse NIH-3T3 cells grown on a soft agarose medium. Colony formation based on anchoring independent cell growth is a feature of malignant transformed cells. These factors did not exhibit colony formation activity but repression of the ras oncogene induced this activity. PR-SET7 suppressed RASval12-mediated colony formation through methyltransferase activity. These results suggest that H2a.z and Pr-set7 that are induced during hepatocarcinogenesis may function as carcinogenesis suppressors.
- 公益社団法人 日本薬学会の論文
著者
-
OSADA Shigehiro
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University
-
GOMITA Urara
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University
-
IMAGAWA Masayoshi
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University
-
Hashizume Hiroshi
Department of Chemistry, University of Shizuoka
-
Gomita Urara
Department Of Molecular Biology Graduate School Of Pharmaceutical Sciences Nagoya City University
-
Osada Shigehiro
Department Of Molecular Biology Graduate School Of Pharmaceutical Sciences Nagoya City University
-
Hashizume Hiroshi
Deparment Of Chemistry Shizuoka University
-
Imagawa Masayoshi
Department Of Environmental Biochemistry Faculty Of Pharmaceutical Sciences Osaka University
-
Osada Shigehiro
Department of Environmental Biochemistry, Faculty of Pharmaceutical Sciences, Osaka University
関連論文
- Toxicity of Nickel Compounds Mediated by HTZ1, Histone Variant H2A.Z, in Saccharomyces cerevisiae(Molecular and Cell Biology)
- TC10-Like/TC10βLong Regulates Adipogenesis by Controlling Mitotic Clonal Expansion
- Gelsolin, an Actin Regulatory Protein, is Required for Differentiation of Mouse 3T3-L1 Cells into Adipocytes
- Promotion of Anchorage-independent Growth by Cytoplasmic and Nuclear Histone Deacetylase 9
- His151 and His296 Are the Acid-Base Catalytic Residues of Bacillus cereus Sphingomyelinase in Sphingomyelin Hydrolysis
- Cloning and Characterization of a cDNA Encoding the Histone Acetyltransferase Monocytic Leukemia Zinc Finger Protein (MOZ) in the Rat
- 1-Chloro-2,4-Dinitrobenzene Stimulates the Estrogenic Activity in MCF-7 Cells
- Actin Accumulation in Physarum polycephalum Nuclei by Heat Shock
- Digestive Enzymes from the Upper Lip of a Bioluminescent Ostracod, Vargula hilgendorfii
- Specific Detection of Lysophosphatidic Acids in Serum Extracts by Tandem Mass Spectrometry
- Repression of the Promoter Activity Mediated by Liver Receptor Homolog-1 through Interaction with Ku Proteins
- Fad24 Causes Hyperplasia in Adipose Tissue and Improves Glucose Metabolism
- Identification of a Novel Vitamin D Response Element from the Rat Genome^1
- FAD24, a Regulator of Adipogenesis, is Required for the Regulation of DNA Replication in Cell Proliferation(Molecular and Cell Biology)
- Hydrodynamic and Spectroscopic Studies on the Interaction of Purple Membrane Particles with Sodium Dodecyl Sulphate
- Isolation of Genes Regulated by Peroxisome Proliferator-Activated Receptor γ(PPARγ) by Two-Dimensional Electrophoresis and Mass Spectrometry
- Spinal Glial Activation and Cytokine Expression After Lumbar Root Injury in the Rat
- Physico-chemical Characterization of Red Membrance from Halobacterium halobium
- Development of Standardized in Vitro Assay System for Estrogen Receptors and Species Specificity of Binding Ability of 4-Nonylphenol and p-Octylphenol
- Development of a Competitive Enzyme Immunoassay for Detection of Capacity of Chemicals to Bind Quail Estrogen Receptor α and β
- The Use of Cultured Bone Marrow Cells in Type I Collagen Gel and Porous Hydroxyapatite for Posterolateral Lumbar Spine Fusion
- A Prospective Randomized Study of Clinical Outcomes in Patients With Cervical Compressive Myelopathy Treated With Open-Door or French-Door Laminoplasty
- Osteogenic Protein-1 (Osteogenic Protein-1/Bone Morphogenetic Protein-7) Inhibits Degeneration and Pain-Related Behavior Induced by Chronically Compressed Nucleus Pulposus in the Rat
- Lumbar Sagittal Balance Influences the Clinical Outcome After Decompression and Posterolateral Spinal Fusion for Degenerative Lumbar Spondylolisthesis
- Evaluation of Carriers of Bone Morphogenetic Protein for Spinal Fusion
- Glu-53 of Bacillus cereus Sphingomyelinase Acts as an Indispensable Ligand of Mg^ Essential for Catalytic Activity
- Conformational Change of Boundary Lipids Surrounding the Rhodopsin of Purple Membrane by Conjugation of Sodium Dodecyl Sulphate
- Nuclear Factor 1 Family Proteins Bind to the Silencer Element in the Rat Glutathione Transferase P Gene^1
- Histone Methyltransferase PR-Set7 and Histone Variant H2A.Z, Induced during Hepatocarcinogenesis, Repress the Promoter Activity of the Tumor Marker Gene and the Ras-Induced Colony Formation Activity
- Functional Analysis of Zinc Finger Proteins That Bind to the Silencer Element in the Glutathione Transferase P Gene
- Identification of Zinc Finger Proteins Bound to a Silencer Region in the Rat Glutathione Transferase P Gene
- Gene Expression of CCAAT/Enhancer-Binding Protein δ Mediated by Autoregulation Is Repressed by Related Gene Family Proteins
- Genomic Organization of the Rat Nuclear Factor I-A Gene
- CCAAT/Enhancer-Binding Protein δ Gene Expression Is Mediated by APRF/STAT3^1
- Conformational Studies on the Acidic Proteins of Rat Liver Ribosomal Large Subunit: Fluorometric Evidences for Heterodimeric Interaction of P1 and P2 Ribosomal Proteins
- Changes in the extracellular matrix on the surface of sintered bovine bone implanted in the femur of a rabbit:An immunohistochemical study
- Factor for Adipocyte Differentiation 158 Gene Disruption Prevents the Body Weight Gain and Insulin Resistance Induced by a High-Fat Diet
- Evaluation of Ability of Chemicals to Bind Frog (Xenopus laevis) Estrogen Receptor by in Vitro Binding Assay
- Dynamic Aspects of Conformational Regulation of the Oncogene Product, c-Myb Protein, by Specific DNA-Binding
- A Prunus mume Extract Stimulated the Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells
- Effective Extraction and Analysis for Lysophosphatidic Acids and Their Precursors in Human Plasma Using Electrospray Ionization Mass Spectrometry
- Chromatographic Characterization of Nuclear and Cytoplasmic Polyadenylate-rich RNA in immunized and nonimmunized Mouse Lymphocytes
- Micro-scale Chromatography of Nucleic Acids on a Methylated Albumin-Kieselghur Column and its Application to the Analysis of Poly(A)^+ RNA and Two Dimensional Identification of Nucleic Acids
- Carbon-13 NMR Spectrometric Characterization of Egg White Lysozyme Carboxyl Groups in Relation to the Binding Environment for N-acetyl Glucosamine
- Discontinous Degradation of Supramolecular Organization Comprising Light Harvesting Chlorophyll a/b-protein and Chlorophyll Protein Complex 1 by Sodium Dodecyl Sulphate
- Factors related to spinal Instability after decompression surgery without fusion for lumbar degenerative spondylolisthesis
- A Prunus mume Extract Stimulated the Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells
- Variability and Adaptation of Light Harvesting Chlorophyll a/b Protein Complex(LHC) in a green algae, Chlorella ellipsoidea, Depending on the Irradiation of Light
- Transformation and DNA Synthesis of Mouse Platelets Stimulated with Concanavalin A
- Inactivation of Penicillium digitatum Spores by a High-Density Ground-State Atomic Oxygen-Radical Source Employing an Atmospheric-Pressure Plasma
- Interactions of Thyroid Hormone Receptor with Ku Proteins and Interleukin Enhancer Binding Factor 3 Modulate the Promoter Activity of Thyroid-Stimulating Hormone Alpha
- Experimental study of carriers of bone morphogenetic protein used for spinal fusion