A Prunus mume Extract Stimulated the Proliferation and Differentiation of Osteoblastic MC3T3-E1 Cells

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概要

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• Osteoporosis is a serious disease caused by decreased bone mass. There is constant matrix remodeling in bones, by which bone formation is performed by osteoblastic cells, whereas bone resorption is accomplished by osteoclast cells. We investigated the effect of a Japanese apricot (Prunus mume SIBE. et ZUCC.) extract on the proliferation and osteoblastic differentiation in pre-osteoblastic MC3T3-E1 cells. An alkaline phosphatase (ALP) activity assay, cell proliferation assay, alizarin red staining and expression analysis of osteoblastic genes were carried out to assess the proliferation and osteoblastic differentiation. The water-soluble fraction of Prunus mume (PWF) increased the ALP activity, cell proliferation and mineralization. The gene expression of osteopontin and bone morphogenetic protein-2, which are markers in the early period of osteoblastic differentiation, were significantly enhanced by the PWF treatment. PWF therefore stimulated the proliferation and osteoblastic differentiation of cells and may have potential to prevent osteoporosis.

• 2011-10-23

著者

• Inada Ken-ichi

  Department Of Pathology Fujita Health University School Of Medicine

• Inada Ken-ichi

  愛知県がんセンター研究所 腫瘍病理学部

• Inada Ken-ichi

  Division Of Oncological Pathology Aichi Cancer Center Research Institute

• 稲田 健一

  Department Of Pathology Fujita Health University School Of Medicine

• Nakamura Misa

  Department of Pathology, Wakayama Medical University

• Okuno Yoshiharu

  Second Dep. Of Pathology Wakayama Medical Univ.

• Okuno Yoshiharu

  Department Of Applied Chemistry Faculty Of Science And Engineering Kinki University

• Utsunomiya Hirotoshi

  Department Of Pathology School Of Medicine Tokai University

• YOSHIDA Munehito

  Department of Orthopedic Surgery, Wakayama Medical University

• Hashizume Hiroshi

  Deparment Of Chemistry Shizuoka University

• Kono Ryohei

  Department of biotechnological sciences, school of biology-oriented science and technology, Kinki un

• Yoshida Munehito

  Department Of Orthopaedic Surgery Wakayama Medical College

• TOKUDA Akihiko

  Department of Strategic Surveillance for Functional Food and Comprehensive Traditional Medicine, Wak

• Utsunomiya Hirotoshi

  Department Of Strategic Surveillance For Functional Food And Comprehensive Traditional Medicine Waka

• Nakamura Misa

  Department Of Rehabilitation Osaka Kawasaki Rehabilitation University

• Kono Ryohei

  Department Of Strategic Surveillance For Functional Food And Comprehensive Traditional Medicine Waka

• Hashizume Hiroshi

  Department Of Orthopedic Surgery Wakayama Medical University

• Tokuda Akihiko

  Department Of Strategic Surveillance For Functional Food And Comprehensive Traditional Medicine Waka

• Inada Ken-ichi

  Department of Pathology, Fujita Health University School of Medicine

• NAKAMURA Misa

  Department of Biological Functions and Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Techonogy

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