Evaluation of Ability of Chemicals to Bind Frog (Xenopus laevis) Estrogen Receptor by in Vitro Binding Assay
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概要
- 論文の詳細を見る
The in vitro binding assay seems to be a useful first screening method for endocrine disrupting chemicals. Various methods have been developed and applied to the testing of chemicals. Since these assays should be applied to estrogen receptors (ER) of not only humans but also wildlife, we previously developed a standardized in vitro binding assay system for human, quail, Japanese medaka, and Xenopus laevis ERs using a competitive enzyme immunoassay. Since that was a first report on an in vitro binding assay system for Xenopus ER_α, and the capacities of chemicals to bind Xenopus ER_α were not tested yet, we here evaluated the ability of 20 test chemicals, which were selected by the Ministry of the Environment of Japan, to bind Xenopus ER_α. Of these, 4-nonylphenol, p-octylphenol and bisphenol A had relatively high binding capacity, and these results are similar to those obtained previously in quail ER_α.
- 公益社団法人日本薬学会の論文
- 2004-12-01
著者
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Kunimoto Manabu
National Institute For Environmental Studies:department Of Public Health School Of Pharmaceutical Sc
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Kunimoto Manabu
The National Institute For Environmental Studies Tsukuba
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Nishizuka Makoto
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University
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Kunimoto M
National Institute For Environmental Studies:department Of Public Health School Of Pharmaceutical Sc
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Kunimoto Masaaki
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University
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Nishizuka M
Department Of Molecular Biology Graduate School Of Pharmaceutical Sciences Nagoya City University
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Nishizuka Makoto
Department Of Molecular Biology Graduate School Of Pharmaceutical Sciences Nagoya City University
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Imagawa Masayoshi
Department Of Environmental Biochemistry Faculty Of Pharmaceutical Sciences Osaka University
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Suzuki Eiko
Department Of Developmental Medical Sciences Institute Of International Health Graduate School Of Me
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Suzuki Eiko
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University
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