Methylmercury-Mediated Down-Regulation of mtHSP70 and Phospholipase A_2 mRNA Expression in Human Neuroblastoma NB-1 Cells Identified by cDNA Macroarray Analysis
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概要
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Methylmercury (MeHg) is a well-known environmental pollutant that causes severe neurological damage, especially in the developing nervous system. When human neuroblastoma NB-1 cells were treated with MeHg at sublethal concentrations, down-regulation of two apoptosis-associated genes, mtHSP70 (GRP75/PBP74/mtHSP75/HSPA9B/mortalin) and phospholipase A_2 (PLA2), was identified using the cDNA macroarray technique. These observations were further confirmed by RT-PCR and Northern blotting experiments. Northern blotting data also demonstrated that only groups III and VI B of PLA2 were down-regulated, while group IIE remained unchanged. These results suggest that the mtHSP70 and PLA2 may be involved in the MeHg-mediated degeneration of neuronal cells.
- 公益社団法人日本薬学会の論文
- 2002-08-01
著者
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Kunimoto Manabu
National Institute For Environmental Studies:department Of Public Health School Of Pharmaceutical Sc
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Kunimoto Manabu
The National Institute For Environmental Studies Tsukuba
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Kunimoto Manabu
Regional Environment Division National Institute For Environmental Studies:department Of Public Heal
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Kunimoto Manabu
Regional Environment Division National Institute For Environmental Studies
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Pramanik Rocky
National Institute For Environmental Studies
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Kunimoto M
National Institute For Environmental Studies:department Of Public Health School Of Pharmaceutical Sc
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Ishido Masami
Regional Environment Division, National Institute for Environmental Studies
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Pramanik Rocky
Regional Environment Division, National Institute for Environmental Studies
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Ishido M
Environmental Risk Res Program National Inst. For Environmental Studies
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Ishido Masami
Regional Environment Division National Institute For Environmental Studies
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