Acute Cytotoxic Effects of Mercuric Compounds in Cultured Astrocytes Prepared from Cerebral Hemisphere and Cerebellum of Newborn Rats(Miscellaneous)

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概要

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• We investigated acute cytotoxic effects and Hg accumulation after exposure to methylmercury (MeHg) or Hg^<2+> in the presence or absence of serum in cultured astrocytes prepared from the cerebral hemisphere or cerebellum of newborn rats. Dose-related changes in viable cell numbers after exposure to mercuric compounds were not different between astrocytes from both regions under the specified conditions. Accumulation of each compound for 3h was similar in both astrocytes but that for 24h became different, especially that of Hg^<2+>. In both astrocytes, susceptibility to the respective compounds was higher in the order of those exposed immediately after, without, and 24h after changing the serum-containing medium to a serum-free defined medium (SFDM). Accumulation for 3h was higher in the respective astrocytes exposed to MeHg or Hg^<2+> immediately after being maintained in SFDM than in those exposed 24h after. These results suggest that accumulation of mercuric compounds up to 3h strongly correlates with susceptibility, at least when maintained in SFDM. Astrocytic morphology changed to a satellite shape after the medium change to SFDM particularly in cerebellar astrocytes but only a few in cerebral hemisphere astrocytes, and it was reverted to a polygonal shape by MeHg but not Hg^<2+> at 3μM. The present results suggest that although some properties such as morphological changes and Hg accumulation are different between cerebral hemisphere and cerebellar astrocytes, these differences are not simply reflected by susceptibility to the acute cytotoxicity of mercuric compounds.

• 公益社団法人日本薬学会の論文
• 2005-12-01

著者

• Adachi Tatsumi

  Regional Environment Division National Institute For Environmental Studies:department Of Basic Medic

• Kunimoto Manabu

  Regional Environment Division National Institute For Environmental Studies:department Of Public Heal

• Kunimoto Manabu

  Regional Environment Division National Institute For Environmental Studies

関連論文

• Effect of Dominant-Negative Expression of L1 Cytoplasmic Domain on the Localization of Brain Ankyrins in Human Neuroblastoma NB-1 Cells
• Methylmercury-Mediated Down-Regulation of mtHSP70 and Phospholipase A_2 mRNA Expression in Human Neuroblastoma NB-1 Cells Identified by cDNA Macroarray Analysis
• Regulation of Cell Fate by Cadmium and Zinc
• Acute Cytotoxic Effects of Mercuric Compounds in Cultured Astrocytes Prepared from Cerebral Hemisphere and Cerebellum of Newborn Rats(Miscellaneous)
• A Neuron-Specific Isoform of Brain Ankyrin, 440 kD Ankyrin_B, As a Useful Tool in Neurobiology and Neurotoxicology
• Erratum: Acute Cytotoxic Effects of Mercuric Compounds in Cultured Astrocytes Prepared from Cerebral Hemisphere and Cerebellum of Newborn Rats [Biol. Pharm. Bull. 28(12): 2308-2311 (2005)]

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