FAD24, a Regulator of Adipogenesis, is Required for the Regulation of DNA Replication in Cell Proliferation(Molecular and Cell Biology)
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概要
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A novel gene, factor for adipocyte differentiation 24 (fad24), promotes adipogenesis by controlling DNA replication early on during a stage referred to as mitotic clonal expansion (MCE). MCE is considered distinct from the proliferation of pre-confluent cells, so we investigated the role of fad24 in the process. First, the expression of fad24 was examined in pre-confluent and post-confluent 3T3-L1 preadipocytes, NIH-3T3 fibroblasts, and C2C12 myoblasts. fad24 was strongly expressed in the pre-confluent cells. The knockdown of fad24 by RNA interference impaired the ability of the pre-confluent cells to proliferate. Moreover, bromodeoxyuridine labeling and chromatin immunoprecipitation experiments indicated that the knockdown inhibited DNA synthesis by preventing the recruitment of histone acetyltransferase binding to ORC1 (HBO1), a component of the pre-replicative complex, to origins. fad24 plays positive roles in the proliferation of pre-confluent cells as well as adipogenesis.
- 2008-06-01
著者
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OSADA Shigehiro
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University
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Osada Shigehiro
Department Of Molecular Biology Graduate School Of Pharmaceutical Sciences Nagoya City University
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Nishizuka Makoto
Department Of Molecular Biology Graduate School Of Pharmaceutical Sciences Nagoya City University
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Imagawa Masayoshi
Department Of Environmental Biochemistry Faculty Of Pharmaceutical Sciences Osaka University
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JOHMURA Yoshikazu
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University
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Osada Shigehiro
Department of Environmental Biochemistry, Faculty of Pharmaceutical Sciences, Osaka University
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