Isolation of Genes Regulated by Peroxisome Proliferator-Activated Receptor γ(PPARγ) by Two-Dimensional Electrophoresis and Mass Spectrometry
スポンサーリンク
概要
- 論文の詳細を見る
To isolate the genes regulated by peroxisome proliferator-activated receptor γ (PPARγ), we first developed a stable transformant expressing PPARγ. Using cytosol and nuclear fractions prepared from this cell line, we performed two-dimensional electrophoresis. A comparison of the electrophoretic patterns with those from control cells revealed many spots to be up-regulated or down-regulated proteins. Some spots were subjected to mass spectrometric analyses, and triosephosphate isomerase and galectin-3 were identified as proteins down-regulated by PPARγ. Moreover, Northern blot analyses revealed that the expression level of both genes decreased in PPARγ-expressing cells. These results strongly suggest that triosephosphate isomerase and galectin-3 are target genes whose expression is mediated by PPARγ.
- 公益社団法人日本薬学会の論文
- 2004-06-01
著者
-
IMAGAWA Masayoshi
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University
-
Taguchi Ryo
Department of Metabolome, Faculty of Medicine, The University of Tokyo
-
Taguchi Ryo
Department Of Metabolome Faculty Of Medicine The University Of Tokyo
-
Taguchi Ryo
Univ. Tokyo Tokyo Jpn
-
Tsuchiya Saori
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University
-
Arimoto Emi
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University
-
Nishizuka Makoto
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University
-
Arimoto Emi
Department Of Molecular Biology Graduate School Of Pharmaceutical Sciences Nagoya City University:la
-
Tsuchiya Saori
Department Of Molecular Biology Graduate School Of Pharmaceutical Sciences Nagoya City University
-
Nishizuka Makoto
Department Of Molecular Biology Graduate School Of Pharmaceutical Sciences Nagoya City University
-
Imagawa Masayoshi
Department Of Molecular Biology Graduate School Of Pharmaceutical Sciences Nagoya City University
-
Imagawa Masayoshi
Department Of Environmental Biochemistry Faculty Of Pharmaceutical Sciences Osaka University
-
Taguchi Ryo
Department Of Medicine And Molecular Science Gunma University Graduate School Of Medicine
関連論文
- Efficient Identification and Quantification of Peptides Containing Nitrotyrosine by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry after Derivatization
- Toxicity of Nickel Compounds Mediated by HTZ1, Histone Variant H2A.Z, in Saccharomyces cerevisiae(Molecular and Cell Biology)
- TC10-Like/TC10βLong Regulates Adipogenesis by Controlling Mitotic Clonal Expansion
- Gelsolin, an Actin Regulatory Protein, is Required for Differentiation of Mouse 3T3-L1 Cells into Adipocytes
- 2 Metabolome analysis revealed the inherent capacity of heart to acquire enhanced tolerance to oxidative stress via metabolic reprogramming(Symposium 7 (SY-07) (M) Molecular Mechanism and Novel Therapeutic Target in Heart Failure,Special Program,The 72nd
- Promotion of Anchorage-independent Growth by Cytoplasmic and Nuclear Histone Deacetylase 9
- His151 and His296 Are the Acid-Base Catalytic Residues of Bacillus cereus Sphingomyelinase in Sphingomyelin Hydrolysis
- Change in the Membranous Lipid Composition Accelerates Lipid Peroxidation in Young Rat Hearts Subjected to 2 Weeks of Hypoxia Followed by Hyperoxia
- Focused lipidomics by tandem mass spectrometry
- Specific Detection of Lysophosphatidic Acids in Serum Extracts by Tandem Mass Spectrometry
- Repression of the Promoter Activity Mediated by Liver Receptor Homolog-1 through Interaction with Ku Proteins
- Fad24 Causes Hyperplasia in Adipose Tissue and Improves Glucose Metabolism
- Identification of a Novel Vitamin D Response Element from the Rat Genome^1
- FAD24, a Regulator of Adipogenesis, is Required for the Regulation of DNA Replication in Cell Proliferation(Molecular and Cell Biology)
- Growth Inhibition, Morphological Change, and Ectoenzyme Release of LLC-PK1 Cells by Phosphatidylinositol-specific Phospholipase C of Bacillus thuringiensis
- Isolation of Genes Regulated by Peroxisome Proliferator-Activated Receptor γ(PPARγ) by Two-Dimensional Electrophoresis and Mass Spectrometry
- Development of Standardized in Vitro Assay System for Estrogen Receptors and Species Specificity of Binding Ability of 4-Nonylphenol and p-Octylphenol
- Development of a Competitive Enzyme Immunoassay for Detection of Capacity of Chemicals to Bind Quail Estrogen Receptor α and β
- Glu-53 of Bacillus cereus Sphingomyelinase Acts as an Indispensable Ligand of Mg^ Essential for Catalytic Activity
- Nuclear Factor 1 Family Proteins Bind to the Silencer Element in the Rat Glutathione Transferase P Gene^1
- Histone Methyltransferase PR-Set7 and Histone Variant H2A.Z, Induced during Hepatocarcinogenesis, Repress the Promoter Activity of the Tumor Marker Gene and the Ras-Induced Colony Formation Activity
- CCAAT/Enhancer-Binding Protein δ Gene Expression Is Mediated by APRF/STAT3^1
- GM1/GD1b/GA1 synthase expression results in the reduced cancer phenotypes with modulation of composition and raft-localization of gangliosides in a melanoma cell line
- Global analysis of triacylglycerols including oxidized molecular species by reverse-phase high resolution LC/ESI-QTOF MS/MS
- Factor for Adipocyte Differentiation 158 Gene Disruption Prevents the Body Weight Gain and Insulin Resistance Induced by a High-Fat Diet
- Evaluation of Ability of Chemicals to Bind Frog (Xenopus laevis) Estrogen Receptor by in Vitro Binding Assay
- Diapause Response to Photoperiod and Temperature in Lepisma saccharina Linnaeus (Thysanura : Lepismatidae)
- Separation and quantification of sn-1 and sn-2 fatty acid positional isomers in phosphatidylcholine by RPLC-ESIMS/MS
- Analysis of oxidized phosphatidylcholines as markers for oxidative stress, using multiple reaction monitoring with theoretically expanded data sets with reversed-phase liquid chromatography/tandem mass spectrometry
- Effective Extraction and Analysis for Lysophosphatidic Acids and Their Precursors in Human Plasma Using Electrospray Ionization Mass Spectrometry
- Increase of oxidant-related triglycerides and phosphatidylcholines in serum and small intestinal mucosa during development of intestinal polyp formation in Min mice
- Attenuated expression of menin and p27^ in an aggressive case of multiple endocrine neoplasia type 1 (MEN1) associated with an atypical prolactinoma and a malignant pancreatic endocrine tumor
- 33rd Annual Meeting of the Japanese Society for Biomedical Mass Spectrometry
- KCNJ5 mutations in aldosterone- and cortisol-co-secreting adrenal adenomas
- Interactions of Thyroid Hormone Receptor with Ku Proteins and Interleukin Enhancer Binding Factor 3 Modulate the Promoter Activity of Thyroid-Stimulating Hormone Alpha