Growth Inhibition, Morphological Change, and Ectoenzyme Release of LLC-PK1 Cells by Phosphatidylinositol-specific Phospholipase C of Bacillus thuringiensis

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概要

• 論文の詳細を見る

• Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus thuringiensis added to a culture of LLC-PK1 cells inhibited cell growth by 40%. In contrast with normal cells, the cells cultured in the presence ote PI-PLC showed needle-like appendages which seemed to have been formbd due to portions of the cell remaining adhered to the culture dish as the cell shrank. When LLC-PK1 cells were treated with PI-PI/C, significant amounts of alkaline phosphatase and alkaline phosphodiesterase I were released specifically from the apical surface of the LLC-PK1 cells. Furthermore, PI-PLC treatment caused a delay of enzyme production and dome formation. These data indicate that glycosyl-phosphatidylinositol (GPI)-anchored proteins on the surface of LLC-PK1 cells are important in cell growth and differentiation. Also, the combined use of LLC-PK1 cells and PI-PLC of B. thuringiensis is effective for investigating the function of GPI-anchor proteins.

• 社団法人日本農芸化学会の論文
• 1997-05-23

著者

• Taguchi Ryo

  Department of Metabolome, Faculty of Medicine, The University of Tokyo

• Taguchi R

  Nagoya City Univ. Nagoya Jpn

• IKEZAWA Hiroh

  Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University

• Taguchi Ryo

  Univ. Tokyo Tokyo

• Ikezawa H

  Nagoya City Univ. Aichi Jpn

• Nakamura Tomoko

  School Of Pharmaceutical Sciences Mukogawa Women S University

• ITAMI Chisako

  Laboratory of Biotchemistry, Faculty of Pharmaceutical Sciences, Mukogawa Women's University

• KIMURA Yukio

  Laboratory of Biotchemistry, Faculty of Pharmaceutical Sciences, Mukogawa Women's University

• NAKABAYASHI Toshikatsu

  Laboratory of Biotchemistry, Faculty of Pharmaceutical Sciences, Mukogawa Women's University

• Taguchi Ryo

  Department Of Metabolome Faculty Of Medicine The University Of Tokyo

• Itami C

  Laboratory Of Biochemistry Faculty Of Pharmaceutical Sciences Mukogawa Women's University

• Nakabayashi T

  School Of Pharmacy And Pharmaceutical Sciences Mukogawa Women's University

• Kimura Yukio

  Laboratory Of Biotchemistry Faculty Of Pharmaceutical Sciences Mukogawa Women's University

• Taguchi Ryo

  Department Of Medicine And Molecular Science Gunma University Graduate School Of Medicine

• Itami Chisako

  Laboratory of Biochemistry Faculty of Pharmaceutical Sciences, Mukogawa Women's University

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