Effect of Quinine Solutions on Intracellular Ca^<+2>Levels in Neuro-2a Cells : Conventional Physiological Method for the Evaluation of Bitterness(Miscellaneous)

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The purpose of the present study was to examine the effect of quinine on intracellular Ca^<2+>([Ca^<2+>]_i) levels in cultured neuro-2a cells, and to investigate the possibility of using [Ca^<2+>]_i levels to predict the bitterness of quinine solutions. [Ca^<2+>]_i levels in neuro-2a cells increased following stimulation by quinine in a concentration-related manner. There was a good linear correlationship between the quinine-induced increase in [Ca^<2+>]_i levels increase and the bitterness scores of the quinine solutions as assessed in human gustatory sensation tests (r^2=O.918). The quinine-induced increase in [Ca^<2+>]_i levels was inhibited by thapsigargin (an inhibitor of the Ca^<2+> pump into intracellular stores), U73122 (an inhibitor of phospholipase C) and ω-conotoxin (an N-type Ca^<2+>-channel blocker), but not by nifedipine (an L-type Ca^<2+> -channel blocker).
2003-11-01