An Attempt to Evaluate the Effect of Vitamin K_3 Using as an Enhancer of Anticancer Agents(Pharmacology)

概要

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The possibility of vitamin K_3 (VK_3) as an anticancer agent was assessed. VK_3 dose-dependently diminished the cell viability (measured as esterase activity) with IC_<50> of 13.7 μM and Hill coefficient of 3.1 in Hep G2 cells. It also decreased the population of S phase and arrested cell cycle in the G_2/M phase in a dose-dependent manner. G_2/M arrest was regulated by the increment of cyclin A/cdk1 and cyclin A/cdk2 complex, and contrasting cyclin B/cdk1 complex decrease. Finally, combined application demonstrated that VK_3 significantly enhanced the cytotoxicity of etoposide, a G_2 phase-dependent anticancer agent, whereas it reduced the cytotoxic activity of irinotecan, a S phase-dependent agent. These findings suggest that VK_3 induces G_2/M arrest by inhibition of cyclin B/cdk1 complex formation, and is thus useful as an enhancer of G_2 phase-dependent drugs in hepatic cancer chemotherapy.
公益社団法人日本薬学会の論文
2008-06-01