Release of Ectoenzymes from Small Intestine Brush Border Membranes of Mice by Phospholipases

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概要

• 論文の詳細を見る

• This study investigated ectoenzyme release from small intestine brush border membranes (duodenum and jejunum, Preparation A; ileum, Preparation B) of mice by the action of phosphatidylinositol-specific phospholipase C or glycosyl-phosphatidylinositol-specific phospholipase D. Most of the alkaline phosphatase was solubilized from Preparation A, but about 60% was released from Preparation B. As for alkaline phosphodiesterase I activity, 15 and 10% were released from Preparations A and B, respectively. With Preparation B, octylglucoside treatment followed by phosphatidylinositol-specific phospholipase C or glycosyl-phosphatidylinositol-specific phospholipase D completely solubilized the alkaline phosphatase activity. However, this treatment did not change the ratio of release of alkaline phosphodiesterase I from Preparation A or B. These results indicate that the resistance to alkaline phosphatase found in Preparation B is due to hindered accessibility of the bonding splitting by phosphatidylinositol-specific phospholipase C and not to a modified glycosyl-phosphatidylinositolanchor.

• 社団法人日本農芸化学会の論文
• 1997-02-23

著者

• Taguchi R

  Nagoya City Univ. Nagoya Jpn

• Taguchi Ryo

  Univ. Tokyo Tokyo

• Ikezawa H

  Nagoya City Univ. Aichi Jpn

• Nakamura Tomoko

  School Of Pharmaceutical Sciences Mukogawa Women S University

• ITAMI Chisako

  Laboratory of Biotchemistry, Faculty of Pharmaceutical Sciences, Mukogawa Women's University

• NAKABAYASHI Toshikatsu

  Laboratory of Biotchemistry, Faculty of Pharmaceutical Sciences, Mukogawa Women's University

• TAGUCHI Ryo

  Laboratory of Biochemistry Faculty of Pharmaceutical Sciences, Mukogawa Women's University

• IKEZAWA Hiroh

  Laboratory of Biochemistry Faculty of Pharmaceutical Sciences, Mukogawa Women's University

• Itami C

  Laboratory Of Biochemistry Faculty Of Pharmaceutical Sciences Mukogawa Women's University

• Nakabayashi T

  School Of Pharmacy And Pharmaceutical Sciences Mukogawa Women's University

• Ikezawa Hiroh

  Laboratory of Biochemistry Faculty of Pharmaceutical Sciences, Mukogawa Women's University

• Itami Chisako

  Laboratory of Biochemistry Faculty of Pharmaceutical Sciences, Mukogawa Women's University

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