Preparation and Characterization of Polylactic Acid Microspheres Containing Bovine Insulin by a w/o/w Emulsion Solvent Evaporation Method
スポンサーリンク
概要
- 論文の詳細を見る
The objective of this study was to produce polylactic acid (PLA) microspheres containing bovine insulin as a sparingly water soluble model drug using a water-in-oil-in-water (w/o/w) emulsion solvent evaporation method. The preparative conditions were optimized. Employment of smaller internal aqueous phase volume (50 μl or 100 μl) in the manufacturing process, resulted in the high loading efficiency (over 95% of theoretical insulin loading efficiency). The addition of 10% (w/v) NaCl to the external aqueous phase (0.5% polyvinyl alcohol solution) reduced loading efficiency compared to the case where no NaCl was added to the external phase. The mean volume diameter for prepared PLA microspheres was in the region of 15-25 μm in all cases. PLA microspheres containing 5% and 10% insulin theoretically exhibited burst release in the initial stage. After a three week dissolution test, the surface of the microspheres became more porous due to the degradable characteristics of PLA polymer itself. Nevertheless, about 80% of the insulin still remained undegraded in PLA microspherees. Finally, insulin-loaded PLA microspheres (corresponding to 4 I.U. insulin) were administered to normal rats subcutaneously, and the pharmacological effect (a decrease in serum glucose level) was demonstrated.
- 公益社団法人日本薬学会の論文
- 1997-09-15
著者
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MATSUYAMA KENJI
Faculty of Pharmaceutical Sciences, Kyushu University
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Matsuyama Kenji
Faculty Of Pharmaceutical Sciences Kyushu University
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Matsuyama Kenji
School Of Pharmaceutical Sciences Mukogawa Women's University
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Matsuyama Kenji
Department Of Clinical Pharmacy Faculty Of Pharmaceutical Sciences Mukogawa Women's University
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NAGAREYA Noriko
Faculty of Pharmaceutical Sciences, Mukogawa Women's University
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Nishikata Mayumi
Faculty Of Pharmaceutical Sciences Mukogawa Women's University
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UCHIDA Takahiro
Faculty of Pharmaceutical Sciences, Mukogawa Women's University
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SAKAKIBARA Sadako
Faculty of Pharmaceutical Sciences, Mukogawa Women's University
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Nakai Aki
School Of Pharmaceutical Sciences Mukogawa Women's University
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Nishikata M
School Of Pharmaceutical Sciences Mukogawa Women's University
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Uchida Takahiro
Faculty Of Pharmaceutical Sciences Kyushu University
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Konishi Yuriko
Faculty Of Pharmaceutical Sciences Mukogawa Women's University
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Yoshida K
Unit Of Chemistry Faculty Of Engineering And High Technology Research Center Kansai University
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YOSHIDA Kazushi
Teikoku Zouki Pharmaceutics
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NAKAI Aki
Faculty of Pharmaceutical Sciences, Mukogawa Women's University
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Sakakibara S
Faculty Of Pharmaceutical Sciences Mukogawa Women's University
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Nagareya N
Mukogawa Women's Univ. Nishinomiya City Jpn
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Nagareya Noriko
Faculty Of Pharmaceutical Sciences Mukogawa Women's University
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Sakakibara Sadako
Faculty of Pharmaceutical Sciences, Mukogawa Women's University
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