Integrins α2β1, α5β1 and αvβ5 Are Related to Tumor Growth and Metastasis of Non-small Cell Lung Cancer

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Objective: Integrins are related to the growth, invasion, and metastasis of cancer cells, and lung cancer cells express multiple intcgrin subunits. This study was designed to determine the integrin subunits which play major roles in the growth and metastasis of non-small cell lung cancer(NSCLC). Methods: NSCLC cell lines EBC-1, LK-2, LC-1F, PC-14, PC-3, VMRC-LCD and 1A-5 were transplanted into athymic mice both subcutaneously and intravenously. Subcutaneous tumor sizes were measured twice a week. Carcinoma cells from evident metastatic lesions were obtained and cultured, The patterns of integrin subunit expression were cvaluated using immunofluorescence flowcytometric analysis. Results: Maximal subcutaneous tumor size in the exponential growth phase was negatively correlated with integrin α5 and β1 expression(p=0.028, p-0.035). Three months after transplantation, carcinoma cells from 11 cvident metastatic leslons were obtained and cultured. Expression of integrins α1, α2, α3, α4, α5, α6, αv, β1, β3, β4 and β5 was evaluated by flowcytometry. Expression of integrins α2, α3, α5, α6, β1, β4 and β5 was commonly detected in original cell lines, The evident changes at the sites of metastasis were increases in α2(p=0.04), αv(p=0.05), β1(p=0.008)and β5(p=0.09)expression. Conclusion: These results suggest that integrintegrins α2β1 and αvβ5 are related to metastasis, and that α5β1 is related to tumor growth of NSCLC
2001-04-20