Myocardial Layer-Specific Effect of Myoblast Cell-Sheet Implantation Evaluated by Tissue Strain Imaging

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概要

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• Background: The implantation of skeletal myoblast (SMB) cell-sheets over the damaged area of a myocardial infarction (MI) has been shown to improve global left ventricular (LV) function through a paracrine effect. However, the regeneration process has not been fully evaluated. We hypothesized that the use of tissue Doppler strain M-mode imaging to assess myocardial layer-specific strain might enable detailed visual evaluation of the regenerative ability of SMBs. Methods and Results: SMBs were cultured on temperature-responsive culture dishes to generate cell-sheets. At 4 weeks after inducing anterior MI, the animals were divided into 2 groups: SMB cell-sheet implantation and sham operation (n=6 in each). A total of 30 cell-sheets (1.5×107 cells/sheet) were placed on the epicardium, covering the infarct and border regions. Subendocardial and subepicardial strain values were measured in the infarct, border, and remote regions by tissue Doppler strain analysis. SMB cell-sheet implantation produced the following major effects: progression of LV remodeling was prevented and global LV ejection fraction increased; the subendocardial strain was significantly greater than the subepicardial strain in the treated border region; vascular density in the subendocardium was significantly higher than in the subepicardium in the treated region; the expression of vascular endothelial growth factor was significantly increased. Conclusions: Tissue Doppler strain analysis allows precise evaluation of the effect of cell-sheet implantation on layer-specific myocardial function.  (Circ J 2013; 77: 1063–1072)

著者

• Nakatani Satoshi

  Department Of Cardiology National Cardiovascular Center

• SHUDO Yasuhiro

  Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine

• MIYAGAWA Shigeru

  Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine

• Saito Atsuhiro

  Department Of Bio-medical Engineering Tokai University

• Kawaguchi Naomasa

  Department Of Molecular Pathology Osaka University Graduate School Of Medicine And Health Science

• Shudo Yasuhiro

  Department Of Cardiovascular Surgery Osaka University

• Sakaguchi Taichi

  Department Of Cardiovascular Surgery Mitsui Memorial Hospital

• Sawa Yoshiki

  Department Of Cardiovascular Medicine Osaka University Graduate School Of Medicine

• Fukushima Satsuki

  Department Of Cardiovascular Surgery National Cardiovascular Center

• Asanuma Toshihiko

  Department Of Health Sciences Division Of Functional Diagnostics Osaka University Graduate School Of

• Matsuura Nariaki

  Department Of Cardiothoracic Surgery The University Of Tokyo

• Okano Teruo

  Advanced Biomedical Engineer and Science, Tokyo Women's Medical University

• Shimizu Tatsuya

  Advanced Biomedical Engineer and Science, Tokyo Women's Medical University

• ASANUMA Toshihiko

  Department of Functional Diagnostics, Osaka University Graduate School of Medicine

• Asanuma Toshihiko

  Department of Health Sciences, Division of Functional Diagnostics, Osaka University Graduate School of Medicine

• Saito Atsuhiro

  Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine

• Nakatani Satoshi

  Department of Health Sciences, Division of Functional Diagnostics, Osaka University Graduate School of Medicine

• Shimizu Tatsuya

  Advanced Biomedical Engineer and Science, Tokyo Women's Medical University

• KAWAGUCHI Naomasa

  Department of Cardiovascular Pathology, Osaka University Graduate School of Medicine and Health Science, Osaka, Japan

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