1-Chloro-2,4-Dinitrobenzene Stimulates the Estrogenic Activity in MCF-7 Cells
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概要
- 論文の詳細を見る
The estrogenic effect of chemicals, including 1-chloro-2,4-dinitrobenzene (CDNB), in the combination with 17β-estradiol (E_2) was screened by a reporter gene assay using breast cancer cells (MCF-7). It was found that CDNB stimulated E_2-induced transcriptional activity of estrogen receptor (ER) and down-regulated both ER protein and mRNA. However, CDNB alone and CDNB metabolite(s) showed no estrogenic activity and no binding activity to ER, suggesting an indirect pathway other than ER. CDNB gave no transcriptional activity for an aryl hydrocarbon receptor (AhR), suggesting no possibility of a pathway through cross-talk between AhR and ER. On the other hand, CDNB enhanced the mitogen activated protein kinase (MAPK) pathway, suggesting estrogenic action via MAPK. These results indicated that CDNB possessed estrogen-like activity in the transcription and regulation of ER though a different pathway from E_2.
- 社団法人日本薬学会の論文
- 2004-12-01
著者
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OSADA Shigehiro
Department of Molecular Biology, Graduate School of Pharmaceutical Sciences, Nagoya City University
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Nishikawa Jun-ichi
大阪大学 薬学研究科生命情報環境科学
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Nishikawa J
Laboratory Of Environmental Biochemistry Graduate School Of Pharmaceutical Sciences Osaka University
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NISHIHARA Tsutomu
Laboratory of Environmental Biochemistry, Graduate School of Pharmaceutical Sciences Osaka Universit
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Nishikawa Jun-ichi
Laboratory Of Environmental Biochemistry Graduate School Of Pharmaceutical Sciences Osaka University
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Osada Shigehiro
Laboratory of Environmental Biochemistry, Graduate School of Pharmaceutical Sciences, Osaka Universi
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Jung Joohee
Laboratory of Environmental Biochemistry, Graduate School of Pharmaceutical Sciences, Osaka Universi
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Ishida Kunie
Laboratory of Environmental Biochemistry, Graduate School of Pharmaceutical Sciences, Osaka Universi
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Jung Joohee
Laboratory Of Environmental Biochemistry Graduate School Of Pharmaceutical Sciences Osaka University
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Ishida Kunie
Laboratory Of Environmental Biochemistry Graduate School Of Pharmaceutical Sciences Osaka University
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Osada Shigehiro
Department Of Molecular Biology Graduate School Of Pharmaceutical Sciences Nagoya City University
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Nishihara Tsutomu
Laboratory Of Environmental Biochemistry Graduate School Of Pharmaceutical Sciences Osaka University
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Nishihara Tsutomu
Laboratory Of Environmental Biochemistry Graduate School Of Pharmaceutical Sciences Osaka University
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