Biotransformation of C-Glucosylisoflavone Puerarin to Estrogenic (3S)-Equol in Co-culture of Two Human Intestinal Bacteria(Pharmacognosy)
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概要
- 論文の詳細を見る
Puerarin and daidzein are the major naturally occurring isoflavones in leguminous plants. These two compounds are metabolized to equol by human intestinal flora. Here we isolated two intestinal bacteria capable of metabolizing puerarin and daidzein, respectively, from human feces. One of them, strain PUE, converted puerarin to daidzein by cleaving a C-glucosyl bond, whereas the other, strain DZE, converted daidzein to equol by reducing a double bond in ring C followed by elimination of an oxo group. Based on the 16S ribosomal RNA gene sequence, strain DZE showed 85% similarity with Eggerthella lenta. Equol produced by strain DZE was identified as (3S)-equol through several analytical methods. Moreover, we obtained (3S)-equol from puerarin by coincubation with strain PUE and DZE. In addition, 5-hydroxyequol was obtained from genistein by incubation with strain DZE.
- 社団法人日本薬学会の論文
- 2008-08-01
著者
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服部 征雄
富山大学和漢医薬学総合研究所
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KAKIUCHI Nobuko
Graduate School of Natural Science and Technology, Kanazawa University
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HATTORI Masao
Institute of Natural Medicine, University of Toyama
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Nishihata Tomohiro
Institute of Natural Medicine, University of Toyama
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Jin Jong-Sik
Institute of Natural Medicine, University of Toyama
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Hattori M
Inst. Of Natural Medicine Univ. Of Toyama
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Hattori Masao
Research Institute For Wakan-yaku (traditional Sino-japanese Medicines) Toyama Medical And Pharmaceu
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Hattori M
Research Institute For Wakan-yaku Toyama Medical And Pharmaceutical University
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Hattori Masao
Department Of Metabolic Engineering Institute Of Natural Medicine Toyama Medical And Pharmaceutical
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Hattori M
Institute Of Natural Medicine University Of Toyama
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Nishinaka T
Osaka Univ. Pharmaceutical Sci. Osaka Jpn
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Jin Jong-sik
Institute Of Natural Medicine University Of Toyama
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Kakiuchi Nobuko
Graduate School Of Natural Science And Technology Kanazawa University
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Hattori Masao
Institute Of Natural Medicine Faculty Of Pharmaceutical Sciences
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Hattori Masao
Metabolic Engineering Institute Of Natural Medicine University Of Toyama
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Kakiuchi N
Graduate School Of Natural Science And Technology Kanazawa University
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Nishihata Tomohiro
Institute Of Natural Medicine University Of Toyama
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Kakiuchi Nobuko
Graduate School Of Natural Sci. And Technol. Kanazawa Univ.
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