Qualitative and Quantitative Analysis of Swertia Herbs by High Performance Liquid Chromatography-Diode Array Detector-Mass Spectrometry (HPLC-DAD-MS)
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概要
- 論文の詳細を見る
We evaluated the composition of Swertia herbs using high performance liquid chromatography-diode array detector-mass spectrometry (HPLC-DAD-MS). Eleven peaks of 6 species were unequivocally identified by comparing their retention times, UV spectra, on-line electrospray ionization mass (ESI-MS) spectra, and collision-induced dissociation mass spectrometry/mass spectrometry (CID-MS/MS) data with those of authentic compounds. We adopted wavelengths of 254nm, 340nm and 230nm to simultaneously determine these 11 compounds. By comparing the overall DAD and total ion current (TIC) profiles of various samples, the 6 species were differentiated in terms of the occurrence and/or relative concentrations of the eleven compounds. Our novel validated HPLC-DAD-MS method not only facilitates quality control and identification of Swertia herbs, but is also applicable to systematic investigations of the distribution of secoiridoids, flavonoids, and xanthones in the genus Swertia.
- 2008-04-01
著者
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WANG Zhigang
Division of Metabolic Engineering, Institute of Natural Medicine, University of Toyama
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MA Chaomei
Division of Metabolic Engineering, Institute of Natural Medicine, University of Toyama
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TANG Shuhan
Division of Metabolic Engineering, Institute of Natural Medicine, University of Toyama
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XIAO Huai
Department of Pharmacy, Dali University
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KAKIUCHI Nobuko
Graduate School of Natural Science and Technology, Kanazawa University
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KIDA Hiroaki
ALPS Pharmaceutical Ind. Co., Ltd.
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HATTORI Masao
Division of Metabolic Engineering, Institute of Natural Medicine, University of Toyama
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Ma Chaomei
Division Of Metabolic Engineering Institute Of Natural Medicine University Of Toyama
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Tang Shuhan
Division Of Metabolic Engineering Institute Of Natural Medicine University Of Toyama
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Wang Zhigang
Division Of Metabolic Engineering Institute Of Natural Medicine University Of Toyama
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Hattori Masao
Division Of Metabolic Engineering Institute Of Natural Medicine University Of Toyama
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Xiao Huai
Department Of Pharmacy Dali University
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Kida Hiroaki
Alps Pharmaceutical Ind. Co. Ltd.
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Kida Hiroaki
Alps Pharmaceutical Ind. Co. Ltd
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Kakiuchi Nobuko
Graduate School Of Natural Science And Technology Kanazawa University
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Hattori Masao
Inst. Of Natural Medicine Univ. Of Toyama
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Kakiuchi Nobuko
Graduate School Of Natural Sci. And Technol. Kanazawa Univ.
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