Two Proteins, Mn^<2+>, and Low Molecular Cofactor Are Required for C-Glucosyl-Cleavage of Mangiferin(Biochemistry)
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概要
- 論文の詳細を見る
C-Glucosides, in which sugars are attached to the aglycone by carbon-carbon bonds, are generally resistant to acid and enzyme hydrolysis. The C-glucosyl bond of mangiferin, a xanthone C-glucoside, was cleaved by anaerobic incubation with a human intestinal bacterium, Bacteroides sp. MANG, to give norathyriol. A cell-free extract obtained by sonication of B. sp. MANG demonstrated cleaving activity for mangiferin to norathyriol by adding NADH, diaphorase, and dithiothreitol. Both high molecular weight (>10k) and low molecular weight (<10k) fractions obtained from the cell-free extract were required for the activity. MnCl_2 was necessary for the activity, but other metal ions were not. By purification of the high molecular weight fraction using DEAE-cellulose and Phenyl Sepharose column chromatography, two fractions, designated as proteins A and B, were separated and required for the activity. Neither protein A nor protein B alone showed any activity. This is the first report describing a C-glucosyl-cleaving enzyme from human intestinal bacterium that seems to involve a novel enzyme mechanism.
- 社団法人日本薬学会の論文
- 2005-11-01
著者
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NAKAMURA Norio
富山医科薬科大学和漢薬研究所
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Nakamura N
Institute Of Natural Medicine University Of Toyama
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Nakamura Norio
Institute Of Natural Medicine University Of Toyama
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HATTORI Masao
Institute of Natural Medicine, University of Toyama
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AKAO Teruaki
Faculty of Pharmaceutical Sciences, University of Toyama
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Nakamura N
Faculty Of Pharmaceutical Sciences Doshisha Women's College Of Liberal Arts
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Akao Teruaki
Faculty Of Pharmaceutical Sciences University Of Toyama
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Akao Teruaki
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Akao Teruaki
Graduate School Of Medicine And Pharmaceutical Sciences Univ. Of Toyama
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Akao Teruaki
Department Of Enzymatic Physiology Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceuti
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Hattori M
Inst. Of Natural Medicine Univ. Of Toyama
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Hattori Masao
Research Institute For Wakan-yaku (traditional Sino-japanese Medicines) Toyama Medical And Pharmaceu
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Hattori M
Research Institute For Wakan-yaku Toyama Medical And Pharmaceutical University
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Hattori Masao
Department Of Metabolic Engineering Institute Of Natural Medicine Toyama Medical And Pharmaceutical
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SANUGUL Kanjana
Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
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Nakamura N
Institute Of Natural Medicine Toyama Medical And Pharmaceutical University
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Hattori M
Institute Of Natural Medicine University Of Toyama
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Hattori Masao
Institute Of Natural Medicine Faculty Of Pharmaceutical Sciences
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Hattori Masao
Metabolic Engineering Institute Of Natural Medicine University Of Toyama
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Akao Teruaki
Institute Of Natural Medicine Toyama Medical
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Nakamura Norio
Institute Of Natural Medicine
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Sanugul Kanjana
Institute Of Natural Medicine Toyama Medical And Pharmaceutical University
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