Development of a Simple HPLC Method for Determination of Paeoniflorin-Metabolizing Activity of Intestinal Bacteria in Rat Feces
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概要
- 論文の詳細を見る
A simple and reproducible HPLC method for the determination of paeoniflorin (PF)-metabolizing activity of intestinal bacteria in rat feces was developed and validated. Orally administered PF, a major active constituent of Paeoniae Radix, is metabolized into a bioactive compound, paeonimetabolin I (PM-I) by intestinal bacteria. Direct determination of the PF-metabolizing rate into PM-I is hard to achieve by HPLC due to the lack of intense chromophore in PM-I. However, when PF was incubated with Lactobacillus brevis, an intestinal bacterium, in the presence of phenylmercaptan, the metabolizing rate of PF into 8-phenylthio-paeonimetabolin I (PT-PM-I) was found to be equivalent to that of PF into PM-I. Thus, the PF-metabolizing activity of intestinal bacteria in rat feces was determined by measuring the rate of biotransformation of PF into PT-PM-I, which was detected by HPLC at 255 nm. This method can be utilized in the biopharmaceutical study of traditional Chinese formulations containing Paeoniae Radix.
- 公益社団法人日本薬学会の論文
- 2002-09-01
著者
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AKAO Teruaki
Faculty of Pharmaceutical Sciences, University of Toyama
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Tani T
Institute Of Natural Medicine University Of Toyama:21st Century Coe Program University Of Toyama
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Tani T
New Drug Research Department High Quality-life Research Laboratories Bio-medical Division
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Akao Teruaki
Faculty Of Pharmaceutical Sciences University Of Toyama
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Akao Teruaki
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University
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Akao Teruaki
Graduate School Of Medicine And Pharmaceutical Sciences Univ. Of Toyama
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Akao Teruaki
Department Of Enzymatic Physiology Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceuti
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TANI Tadato
Institute of Natural Medicine, University of Toyama
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Tani Tadato
Institute Of Natural Medicine University Of Toyama:21st Century Coe Program University Of Toyama
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He Ju-xiu
Institute Of Natural Medicine University Of Toyama
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〓 忠人
富山医科薬科大学和漢薬研究所和漢薬製剤開発部門:富山医科薬科大学21世紀coeプログラム
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Tani Tadato
Institute Of Natural Medicine Toyama Medical And Pharmaceutical University
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Akao Teruaki
Institute Of Natural Medicine Toyama Medical
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