Indole Alkaloid Production in Callus Cultures of Uncaria rhynchophylla (MIQ.) MiQUEL

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概要

• 論文の詳細を見る

• The effects of growth hormones and nutrients on growth and alkaloid production in callus cultures of Uncaria rhynchophylla (MIQ.) MIQUEL (Rubiaceae) were investigated. Gamborg B5 (B5) medium supplemented with indoleacetic acid (10^<-4>M) and 6-benzylaminopurine (3×10^<-5>M) was optimized both for the growth (1.8g fresh weitht/5 weeks) and alkaloid production (1.73 mg/g dry weight/5 weeks). The influence of sucrose concentration was examined, and 2% sucrose was found to be the most appropriate for the growth and alkaloid production. The effect of the composition of nitrogen sources was examined. Maximal growth and alkaloid production were obtained when 6 mM ammonium chloride and 25mM potassium nitrate were added to the B5 medium. Hirsuteine, hirsutine, 3α-dihydrocadamibine and ursolic acid were isolated from the callus. The 3α-dihydrocadambine concentration of callus was ca. 50-fold higher than that of the hooks and stems of U. rhynchophylla.

• 公益社団法人日本薬学会の論文
• 1996-02-15

著者

• 山崎 勝也

  大阪市立大学医学部生体情報解析学講座

• Yamasaki K

  Faculty Of Pharmaceutical Sciences Teikyo Heisei University

• Tani T

  Institute Of Natural Medicine University Of Toyama:21st Century Coe Program University Of Toyama

• KOHDA Hiroshi

  Institute of Pharmaceutical Sciences, School of medicine, Hiroshima University

• Yamasaki K

  Division Of Medicinal Chemistry Graduate School Of Biomedical Sciences Hiroshima University

• Yamasaki Kazuo

  Institute Of Pharmaceutical Sciences Hiroshima University School Of Medicine

• NAMERA Akira

  Medical Chemistry Laboratory Sumitomo Metal Industries, Ltd.,

• Yamasaki Kazuo

  Institute Of Pharmaceutical Sciences Faculty Of Medicine Hiroshima University

• Namera Akira

  Medicinal Chemistry Labolatory Sumitomo Metal Industries Ltd.

• Koyama Atsuko

  Institute Of Pharmaceutical Sciences Hiroshima University School Of Medicine

• Kohda H

  The Experimental Station Of Medicinal Plant Hiroshima University School Of Medicine

• Kohda Hiroshi

  Institute Of Pharmaceutical Sciences Hiroshima University School Of Medicine

• TANI Tadato

  Medicinal Chemistry Labolatory, Sumitomo Metal Industries Ltd.,

• Tani Tadato

  Medicinal Chemistry Labolatory Sumitomo Metal Industries Ltd.

• Kohda Hiroshi

  Institute Of Pharmaceutical Science Hiroshima University Of Medicine

• Kohda H

  Experimental Station Of Medicinal Plants Graduate School Of Biomedical Sciences Hiroshima University

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