In Vitro Antioxidant Activity of Vietnamese Ginseng Saponin and Its Components

元データ 1998-09-15 公益社団法人日本薬学会

概要

To elucidate the antioxidant action of Vietnamese ginseng saponin against free radial-mediated cellular damage, we examined the effect of Vietnamese ginseng saponin on lipid peroxidation in the mouse brain, liver, and liver microsomes by using two in vitro free radical generating systems (iron ferrous+ascorbic acid and iron ferrous+hydrogen peroxide). Free radical-mediated lipid peroxidation was determined by measuring the endogenous and stimulated accumulation of thiobarbituric acid reactive substance (TBA-RS). Vietnamese ginseng saponin (0.05-0.5 mg/ml), as well as vitamin E, significantly inhibited the formation of TBA-RS in tissue homogenates. Panax ginseng saponin, at the same concentration range as Vietnamese ginseng saponin, also had inhibitory action on free radical-mediated lipid peroxidation. However, majonoside-R2,ginsenoside Rg_1 and ginsenoside-Rb_1,the main saponin components of Vietnamese ginseng saponin fraction, had no effect on lipid peroxidation. These results suggest that Vietnamese ginseng exerts a protective action against free radical-induced tissue injury and that this effect is attributable to minor components rather than the main saponin components tested.

著者

山崎 勝也 大阪市立大学医学部生体情報解析学講座
Huong N Division Of Medicinal Pharmacology Institute Of Natural Medicines Toyama Medical And Pharmaceutical
渡辺 裕司 International Research Center For Traditional Medicine Toyama Prefecture International Health Comple
Watanabe Hiroshi Department of Cardiology, Niigata City General Hospital
Huong Nguyen 富山医科薬科大学和漢薬研究所
MATSUMOTO Kinzo Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical Univers
Yamasaki K Faculty Of Pharmaceutical Sciences Teikyo Heisei University
KASAI Ryoji Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine
Matsumoto Kinzo Div. Of Medicinal Pharmacology Inst. Of Natural Medicine Univ. Of Toyama
Matsumoto Kinzo Department Of Pharmacology Institute Of Natural Medicine:21st Century Coe Program Toyama Medical And
Yamasaki K Division Of Medicinal Chemistry Graduate School Of Biomedical Sciences Hiroshima University
Yamasaki Kazuo Institute Of Pharmaceutical Sciences Hiroshima University School Of Medicine
HOUNG Nguyen Department of Pharmacology, Research Institute for Wakan-Yaku (Oriental Medicines), Toyama Medical a
Yamasaki Kazuo Institute Of Pharmaceutical Sciences Faculty Of Medicine Hiroshima University
Matsumoto Kinzo 富山大学和漢医薬学総合研究所 複合薬物薬理学
Kasai Ryoji Institute Of Pharmaceutical Sciences Faculty Of Medicine Hiroshima University
Watanabe H Division Of Medicinal Pharmacology Institute Of Natural Medicine University Of Toyama
Huong Nguyen Department Of Bioproduction And Environmental Science Faculty Of Agriculture Kyushu University
Watanabe Hiroshi Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers

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