Cytoprotective and Cytotoxic Effects of Curcumin : Dual Action on H_2O_2-Induced Oxidative Cell Damage in NG108-15 Cells

元データ 2003-05-01 社団法人日本薬学会

概要

The ability of curcumin, a natural antioxidant isolated from Curcuma longa, to inhibit hydrogen peroxide (H_2O_2)-induced cell damage in NG108-15 cells was examined. When added simultaneously with 500μM H_2O_2, curcumin (25-10μM) effectively protected cells from oxidative damage. However, when the cells were pretreated with curcumin (25-100μM) for 1.5 h before H_2O_2 exposure, curcumin was unable to inhibit H_2O_2-induced cell damage. Instead, it caused a significant concentration-dependent decrease in cell viability after H_2O_2 exposure. This dual action of curcumin suggests that pretreatment with curcumin by itself did not have any significant effect on the viability of the NG108-15 cells, but it sensitized them to oxidative damage induced by H_2O_2 under our experimental conditions. It appears that these events may not relate to the antioxidant and free radical scavenging activities of curcumin.

著者

Tohda Michihisa 和漢医薬学総合研究所 複合薬物薬理学分野
Tohda Michihisa 富山医科薬科大学和漢薬研究所
Tohda Michihisa Department Of Pharmacology Institute Of Natural Medicine Toyama Medical And Pharmaceutical Universit
渡辺 裕司 International Research Center For Traditional Medicine Toyama Prefecture International Health Comple
Watanabe Hiroshi Department of Cardiology, Niigata City General Hospital
MAHAKUNAKORN Pramote Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical Univers
MURAKAMI Yukihisa Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical Univers
MATSUMOTO Kinzo Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical Univers
Murakami Yukihisa 富山大学和漢医薬学総合研究所 複合薬物薬理学
Mukarami Yukihisa Institute Of Natural Medicine Toyama Medical And Pharmaceutical University
Murakami Yukihisa Division Of Medicinal Pharmacology Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
Murakami Yukihisa Research Institute For Wakan-yaku (traditional Sino-japanese Medicines)
Murakami Yukihisa Department Of Pharmacology Institute Of Natural Medicine:21st Century Coe Program Toyama Medical And
Vajaragupta Opa Department Of Pharmaceutical Chemistry Faculty Of Pharmacy Mahidol University
Murakami Yuko Department Of Bioactive Molecules National Institute Of Infectious Diseases
Tohda Michihisa Department Of Pharmacology Faculty Of Pharmaceutical Sciences Hokkaido University
Matsumoto Kinzo Div. Of Medicinal Pharmacology Inst. Of Natural Medicine Univ. Of Toyama
Matsumoto Kinzo Division Of Medicinal Pharmacology Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
Matsumoto Kinzo Department Of Pharmacology Institute Of Natural Medicine:21st Century Coe Program Toyama Medical And
Matsumoto K Div. Of Medicinal Pharmacology Inst. Of Natural Medicine Univ. Of Toyama
Mahakunakorn Pramote Division Of Medicinal Pharmacology Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
Matsumoto Kinzo 富山大学和漢医薬学総合研究所 複合薬物薬理学
Murakami Y Department Of Bioactive Molecules National Institute Of Infectious Diseases
Murakami Y Toho Univ. Chiba Jpn
渡邉 裕司 富山医科薬科大学生物試験部門
Watanabe Hiroshi Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers

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