Studies on the Activation Mechanisms of Guanylyl Cyclase by Serotonin, Probably through a Novel Subtype of Serotonin Receptor (5-HT_)

元データ 1995-08-15 公益社団法人日本薬学会

概要

Characterization of the serotonin-induced increase in guanosine 3', 5'-cyclic monophosphate (cyclic GMP) was investigated and compared with that induced by atrial natriuretic peptide (ANP) in NG108-15 cells. The cyclic GMP formed by serotonin or ANP was transported in a similar manner to the extracellular medium, although the cyclic GMP formed by bradykinin was not. Serotonin and ANP raised cyclic GMP additively. Serotonin-induced cyclic GMP formation was completely inhibited by pretreatment with 100nM 12-o-tetradecanoylphorbol 13-acetate (TPA), although that induced by ANP was only partialy inhibited and the effects were blocked by pretreatment with staurosporin. In membrane preparations, ANP stimulated cyclic GMP formation in the presence of ATP, but serotonin did not. Serotonin-stimulated cyclic GMP formation was found to occur in neuroblastoma N18TG-2,but not in glioma C6Bu-1. These results suggest that a novel subtype of serotonin receptors (5-HT_<GC>) which stimulates membrane-bound guanylyl cyclase, different from that stimulated by natriuretic peptide, may exist especially in neurons.

著者

Tohda Michihisa 和漢医薬学総合研究所 複合薬物薬理学分野
野村 靖幸 Department of Pharmacological Science, Hokkaido University
関谷 曉子 Department Of Pharmacology Faculty Of Pharmaceutical Sciences Hokkaido University
Sekiya Akiko Department Of Pharmacology Faculty Of Pharmaceutical Sciences Hokkaido University
東田 道久 Department of Pharmacology, Faculty of Pharmaceutical Sciences Hokkaido University
今泉 玲 Department of Pharmacology, Faculty of Pharmaceutical Sciences Hokkaido University
伊藤 奈緒子 Department of Pharmacology, Faculty of Pharmaceutical Sciences Hokkaido University
伊藤 奈緒子 Department Of Pharmacology Faculty Of Pharmaceutical Sciences Hokkaido University
Nomura Yasuyuki Department Of Pharmacology Faculty Of Pharmaceutical Sciences Hokkaido University
今泉 玲 Department Of Pharmacology Faculty Of Pharmaceutical Sciences Hokkaido University

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