The mRNA Expression of Serotonin 2C Subtype Receptors Uncoupled With Inositol Hydrolysis in NG108-15 Cells
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概要
- 論文の詳細を見る
Cell culture systems seem to be useful for clarifying the cellular physiological mechanisms of serotonin 2C subtype receptors (5-HT2CR) and related drug action mechanisms. However, there are still few reports about cells that contain intrinsic 5-HT2CR. This report demonstrates by using RT/PCR that 5-HT2CR mRNA exists in splicing variant forms in NGI08-15 cells. The PCR results using a pair of primers that recognized sequences near the third intracellular loop site showed two neighboring bands at about 500 bp upon electrophoresis in acrylamide gels. The sequence analysis demonstrated that one band was the rat 5-HT2CR sequence and the other one was that of the mouse. Serotonin, however, did not enhance the inositol phosphates formation in NG108-15 cells. It has been reported that post-translational modifications of RNA, splicing and editing, occur at the site of the second intracellular loop domain in 5-HT2CR mRNA. Accordingly, a pair of primers that recognized this site were designed. The molecular size of the PCR product was shorter than that expected based on the sequence of the native 5-HT2CR. The fragment lacked the 95 nucleotides of native 5-HT2CR mRNA. This seems to be the reason why serotonin did not enhance inositol phosphates formation in NG108-15 cells.
- 社団法人 日本薬理学会の論文
- 2002-10-01
著者
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Tohda Michihisa
和漢医薬学総合研究所 複合薬物薬理学分野
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Tohda Michihisa
富山医科薬科大学和漢薬研究所
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Tohda Michihisa
タイ
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Tohda Michihisa
Department Of Pharmacology Institute Of Natural Medicine Toyama Medical And Pharmaceutical Universit
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渡辺 裕司
International Research Center For Traditional Medicine Toyama Prefecture International Health Comple
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Watanabe Hiroshi
Department of Cardiology, Niigata City General Hospital
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Sukma Monrudee
Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical Univers
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Sukma Monrudee
Division Of Medicinal Pharmacology Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
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NOMURA Yasuyuki
Departmen of Pharmacology, Graduate School of Pharmaceutical Sciences, Hokkaido University
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Sukma Monrudee
Department Of Pharmacology Institute Of Natural Medicine Toyama Medical And Pharmaceutical Universit
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Tohda Michihisa
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Hokkaido University
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渡邉 裕司
富山医科薬科大学生物試験部門
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Watanabe Hiroshi
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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