Influence of Chronic Treatment With Imipramine on mRNA Levels in Rat Brain : Elevation of Glyceraldehyde-3-phosphate Dehydrogenase Levels

元データ 1999-12-01 社団法人 日本薬理学会

概要

The differential display method was used to identify the intrinsic factor that changes its mRNA expression level in rat brain after a 14-day oral administration of 20 mg/kg imipramine. The expression of a 180-bp band was markedly enhanced by imipramine. The results of sequencing and a data base search revealed that the isolated clone was glyceraldehyde-3-phosphate dehydrogenase (GAPDH) with a one-base difference. Enhancement of the expression by imipramine was observed in the amygdala. Quantitative PCR showed that imipramine treatment significantly elevated the GAPDH/β-actin ratio in the cortex. These findings suggest that long-term treatment with imipramine stimulates GAPDH mRNA expression.

著者

Tohda Michihisa Department Of Pharmacology Institute Of Natural Medicine Toyama Medical And Pharmaceutical Universit
Watanabe Hiroshi Department of Cardiology, Niigata City General Hospital
Tohda Michihisa Department Of Pharmacology Faculty Of Pharmaceutical Sciences Hokkaido University
Tohda Michihisa Department Of Pharmacology Institute For Wakan-yaku Toyama Medical And Pharmaceutical University
QI Zhao Department of Pharmacology, Institute for Wakan-yaku, Toyama Medical and Pharmaceutical University
Qi Zhao Department Of Pharmacology Institute For Wakan-yaku Toyama Medical And Pharmaceutical University
Watanabe Hiroshi Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers

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