Paeoniflorin, a Major Constituent of Peony Root, Reverses Muscarinic M_1-Receptor Antagonist-Induced Suppression of Long-Term Potentiation in the Rat Hippocampal Slice

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We previously reported that paeoniflorin but not albiflorin, components of peony root, produced ameliorative effects on scopolamine−induced spatial cognitive impairment in rats.In this study, we examined the effects of paeoniflorin and muscarinic receptor antagonists on long−term potentiation(LTP)of population spike recorded from the CA1 region of rat hippocampal slices.Bath applications of an M1− and M2−receptor antagonist scopolamine and a selective M1−receptor antagonist pirenzepine, at a concentration of 10 μM, significantly suppressed LTP, whereas AF−DX116, a selective M2−receptor antagonist, failed to affect it.Paeoniflorin(0.1−1μM), which alone was ineffective on LTP induction, significantly reversed the suppressive effects of scopolamine and pirenzepine(10μM).In contrast, albiflorin(0.1−1μM)had no effect on the scopolamine−induced LTP suppression.These results suggest that paeoniflorin reversal of the muscarinic M1−receptor−mediated inhibition of LTP may be implicated in the ameliorative effect of paeoniflorin on spatial cognitive impairment caused by cholinergic dysfunction.
社団法人日本薬理学会の論文
2000-05-01