Antioxidant and Free Radical-Scavenging Activity of Choto-san and Its Related Constituents(Pharmacology)
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概要
- 論文の詳細を見る
The antioxidant properties of Choto-san and its related constituents such as Chotoko and Choto-san without Chotoko, and phenolic compounds contained in Chotoko such as epicatechin, caffeic, acid and quercetin were evaluated. In the l,l-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging assay, the scavenging activity of Chotoko (IC_<50> 14.3 μg/ml) was found to be higher than that of Choto-san (IC50 206.2 μg/ml) and Choto-san without Chotoko (IC_<50> 244.3μg/ml). Epicatechin (IC_<50> 10.4μM), caffeic acid (IC_<50> 13.8μM), and quercetin (IC_<50> 7.1 μM) also revealed scavenging activity against DPPH radicals. Choto-san (IC_<50> 67.7μg/ml) exhibited stronger inhibitory activity against superoxide anion formation than Choto-san without Chotoko (IC_<50> 92.4 μg/ml) but weaker activity than Chotoko (IC_<50> 18.3 μg/ml). The generation of superoxide anion was also inhibited by epicatechin (IC_<50> 175.2 μM), caffeic acid (IC_<50> 141.7 μM), and quercetin (IC_<50> 18.7 μM). In a hydroxyl radical-scavenging experiment, Choto-san (IC_<50> 2.4 mg/ml), Chotoko (IC_<50> 2.2 mg/ml), Choto-san without Chotoko (IC_<50> 2.8 mg/ml), epicatechin (IC_<50> 3.9 mM), caffeic acid (ICSO 3.6 mM), and quercetin (IC_<50> 1.9 mM) exhibited activity. In NG108-15 cells, when added simultaneously with H_2O_2 (500 μM), Choto-san (250 μg/ml), Chotoko (250 μg/ml), Choto-san without Chotoko (500 μg/ml), epicatechin (200 μ/M), caffeic acid (200 μM), and quercetin (200 μM) effectively prtected cells from oxidative damage. In conclusion, the present results provide evidence that Choto-san acts as an antioxidant and cytoprotective agent against oxidative damage, which is due at least partly to the phenolic compounds contained in Chotoko.
- 2004-01-01
著者
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Tohda Michihisa
和漢医薬学総合研究所 複合薬物薬理学分野
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Tohda Michihisa
富山医科薬科大学和漢薬研究所
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Tohda Michihisa
タイ
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Tohda Michihisa
Department Of Pharmacology Institute Of Natural Medicine Toyama Medical And Pharmaceutical Universit
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渡辺 裕司
International Research Center For Traditional Medicine Toyama Prefecture International Health Comple
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Watanabe Hiroshi
Department of Cardiology, Niigata City General Hospital
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MAHAKUNAKORN Pramote
Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical Univers
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MURAKAMI Yukihisa
Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical Univers
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MATSUMOTO Kinzo
Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical Univers
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Murakami Yukihisa
富山大学和漢医薬学総合研究所 複合薬物薬理学
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Mukarami Yukihisa
Institute Of Natural Medicine Toyama Medical And Pharmaceutical University
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Murakami Yukihisa
Division Of Medicinal Pharmacology Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
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Murakami Yukihisa
Research Institute For Wakan-yaku (traditional Sino-japanese Medicines)
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Murakami Yukihisa
Department Of Pharmacology Institute Of Natural Medicine:21st Century Coe Program Toyama Medical And
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Murakami Yuko
Department Of Bioactive Molecules National Institute Of Infectious Diseases
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Tohda Michihisa
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Hokkaido University
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Matsumoto Kinzo
Div. Of Medicinal Pharmacology Inst. Of Natural Medicine Univ. Of Toyama
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Matsumoto Kinzo
Division Of Medicinal Pharmacology Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
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Matsumoto Kinzo
Department Of Pharmacology Institute Of Natural Medicine:21st Century Coe Program Toyama Medical And
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Matsumoto K
Div. Of Medicinal Pharmacology Inst. Of Natural Medicine Univ. Of Toyama
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Mahakunakorn Pramote
Division Of Medicinal Pharmacology Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
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Matsumoto Kinzo
富山大学和漢医薬学総合研究所 複合薬物薬理学
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Murakami Y
Department Of Bioactive Molecules National Institute Of Infectious Diseases
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Murakami Y
Toho Univ. Chiba Jpn
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渡邉 裕司
富山医科薬科大学生物試験部門
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Watanabe Hiroshi
Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers
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