Antioxidant and Free Radical-Scavenging Activity of Choto-san and Its Related Constituents(Pharmacology)

元データ 2004-01-01

概要

The antioxidant properties of Choto-san and its related constituents such as Chotoko and Choto-san without Chotoko, and phenolic compounds contained in Chotoko such as epicatechin, caffeic, acid and quercetin were evaluated. In the l,l-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging assay, the scavenging activity of Chotoko (IC_<50> 14.3 μg/ml) was found to be higher than that of Choto-san (IC50 206.2 μg/ml) and Choto-san without Chotoko (IC_<50> 244.3μg/ml). Epicatechin (IC_<50> 10.4μM), caffeic acid (IC_<50> 13.8μM), and quercetin (IC_<50> 7.1 μM) also revealed scavenging activity against DPPH radicals. Choto-san (IC_<50> 67.7μg/ml) exhibited stronger inhibitory activity against superoxide anion formation than Choto-san without Chotoko (IC_<50> 92.4 μg/ml) but weaker activity than Chotoko (IC_<50> 18.3 μg/ml). The generation of superoxide anion was also inhibited by epicatechin (IC_<50> 175.2 μM), caffeic acid (IC_<50> 141.7 μM), and quercetin (IC_<50> 18.7 μM). In a hydroxyl radical-scavenging experiment, Choto-san (IC_<50> 2.4 mg/ml), Chotoko (IC_<50> 2.2 mg/ml), Choto-san without Chotoko (IC_<50> 2.8 mg/ml), epicatechin (IC_<50> 3.9 mM), caffeic acid (ICSO 3.6 mM), and quercetin (IC_<50> 1.9 mM) exhibited activity. In NG108-15 cells, when added simultaneously with H_2O_2 (500 μM), Choto-san (250 μg/ml), Chotoko (250 μg/ml), Choto-san without Chotoko (500 μg/ml), epicatechin (200 μ/M), caffeic acid (200 μM), and quercetin (200 μM) effectively prtected cells from oxidative damage. In conclusion, the present results provide evidence that Choto-san acts as an antioxidant and cytoprotective agent against oxidative damage, which is due at least partly to the phenolic compounds contained in Chotoko.

著者

Tohda Michihisa 和漢医薬学総合研究所 複合薬物薬理学分野
Tohda Michihisa 富山医科薬科大学和漢薬研究所
Tohda Michihisa Department Of Pharmacology Institute Of Natural Medicine Toyama Medical And Pharmaceutical Universit
渡辺 裕司 International Research Center For Traditional Medicine Toyama Prefecture International Health Comple
Watanabe Hiroshi Department of Cardiology, Niigata City General Hospital
MAHAKUNAKORN Pramote Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical Univers
MURAKAMI Yukihisa Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical Univers
MATSUMOTO Kinzo Department of Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical Univers
Murakami Yukihisa 富山大学和漢医薬学総合研究所 複合薬物薬理学
Mukarami Yukihisa Institute Of Natural Medicine Toyama Medical And Pharmaceutical University
Murakami Yukihisa Division Of Medicinal Pharmacology Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
Murakami Yukihisa Research Institute For Wakan-yaku (traditional Sino-japanese Medicines)
Murakami Yukihisa Department Of Pharmacology Institute Of Natural Medicine:21st Century Coe Program Toyama Medical And
Murakami Yuko Department Of Bioactive Molecules National Institute Of Infectious Diseases
Tohda Michihisa Department Of Pharmacology Faculty Of Pharmaceutical Sciences Hokkaido University
Matsumoto Kinzo Div. Of Medicinal Pharmacology Inst. Of Natural Medicine Univ. Of Toyama
Matsumoto Kinzo Division Of Medicinal Pharmacology Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
Matsumoto Kinzo Department Of Pharmacology Institute Of Natural Medicine:21st Century Coe Program Toyama Medical And
Matsumoto K Div. Of Medicinal Pharmacology Inst. Of Natural Medicine Univ. Of Toyama
Mahakunakorn Pramote Division Of Medicinal Pharmacology Institute Of Natural Medicine Toyama Medical And Pharmaceutical U
Matsumoto Kinzo 富山大学和漢医薬学総合研究所 複合薬物薬理学
Murakami Y Department Of Bioactive Molecules National Institute Of Infectious Diseases
Murakami Y Toho Univ. Chiba Jpn
渡邉 裕司 富山医科薬科大学生物試験部門
Watanabe Hiroshi Department Of Applied Chemistry And Bioengineering Graduate School Of Engineering Osaka City Univers

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