A New Enzyme Immunoassay for Aconitine and Its Application to Quantitative Determination of Aconitine Levels in Plasma(Pharmacognosy)
スポンサーリンク
概要
- 論文の詳細を見る
A reliable enzyme immunoassay (EIA) method was developed for quantitative determination of aconitine with high sensitivity and specificity. The bovine serum albumin (BSA)- and β-galactosidase (β-Gal) conjugates as immunogens and enzyme-labeled antigens were prepared by coupling of their proteins with succinic acid (short chain length; n=2, where n represents the number of methylene units) and hexadecanedioic acid (long chain length; n=14) hemiesters of benzoylaconine through the respective N-hydroxysuccinimide esters as intermediates. Two types of the BSA-conjugates with short and long chains were repeatedly injected into rabbits to obtain anti-aconitine antisera (As1 and As2, respectively). All combinations of β-Gal-labeled antigens LAg1 (n=2) and LAg2 (n=14) with antisera As1 (n=2) and As2 (n=14) showed high sensitivity to aconitine in a range of 0.1-1.0 ng. Although the combination of LAg2 (n=14) with antiserum As1 (n=2) showed high specificity to aconitine, the combination of LAg2 (n=14) and As2 (n=14) was highly specific to both aconitine and mesaconitine. When aconitine was intravenously administered to rats, the aconitine concentration in their plasma remarkably decreased within the first 60min, and then gradually declined, suggesting a two-compartment pharmacokinetic model in (V_c 0.41 ± 0.09 l/kg, V_<dss> 1.7±0.4 l/kg, CL_<tot> 10±2 ml/min・kg, AUC_<0-4800>±294.3 ng・min/ml). Following oral administration of aconitine to rats at two doses of 0.1 and 1.0 mg/kg b.w., the maximum plasma concentrations (C_<max>) were 0.73±0.08 and 3.3±0.6ng/ml at times of 45±9 and 150±52min, respectively, and the AUC_<0-1440> values were 130±4 and 1600±270 ng・min/ml. The bioavailability (F) of aconitine was determined to be 0.013, where only 1.3% of the aconitine administered orally was absorbed into the body fluid.
- 社団法人日本薬学会の論文
- 2003-09-01
著者
-
Li Yan
Department Of Dermatology Affiliated Union Hospital Tongji Medical College Huazhong University Of Sc
-
NAKAMURA Norio
富山医科薬科大学和漢薬研究所
-
花岡 美代次
金沢大学薬学部
-
Li Yong
Department Of Chemistry Pukyong National University
-
AKAO Teruaki
Faculty of Pharmaceutical Sciences, University of Toyama
-
Li Y
Institute Of Natural Medicine Toyama Medical And Pharmaceutical University
-
Li Yan
Department Of Applied Physics Graduate School Of Engineering Osaka University
-
Akao Teruaki
Faculty Of Pharmaceutical Sciences University Of Toyama
-
Akao Teruaki
Graduate School Of Medicine And Pharmaceutical Sciences Univ. Of Toyama
-
Akao Teruaki
Department Of Enzymatic Physiology Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceuti
-
Hattori Masao
Institute Of Natural Medicine University Of Toyama
-
Hattori Masao
Department Of Metabolic Engineering Institute Of Natural Medicine Toyama Medical And Pharmaceutical
-
TAZAWA Takako
Department of Metabolic Engineering, Institute of Natural Medicine, Toyama Medical and Pharmaceutica
-
ZHAO Huai-qing
Department of Metabolic Engineering, Institute of Natural Medicine, Toyama Medical and Pharmaceutica
-
MESELHY Meselhy
Department of Metabolic Engineering, Institute of Natural Medicine, Toyama Medical and Pharmaceutica
-
NAKAMURA Norio
Department of Metabolic Engineering, Institute of Natural Medicine, Toyama Medical and Pharmaceutica
-
Tazawa T
Department Of Metabolic Engineering Institute Of Natural Medicine Toyama Medical And Pharmaceutical
-
Zhao Huai-qing
Department Of Metabolic Engineering Institute Of Natural Medicine Toyama Medical And Pharmaceutical
-
Nakamura N
Institute Of Natural Medicine Toyama Medical And Pharmaceutical University
-
Meselhy M
Toyama Medical And Pharmaceutical Univ. Toyama Jpn
-
Meselhy Meselhy
Faculty Of Pharmacy Cairo University
-
Meselhy Meselhy
(present Address)faculty Of Pharmacy Cairo University
-
Meselhy Meselhy
Department Of Cell-resources Engineering Institute Of Natural Medicine Toyama Medical And Pharmaceut
-
Li Yan
Key Laboratory Of Materials Physics Institute Of Solid State Physics Chinese Academy Of Sciences
-
Li Y
Graduate School Of Engineering Tottori University
-
Hattori Masao
Department Of Cell-resources Engineering Research Institute For Wakan-yaku (traditional Sino-japanes
-
Li Yan
Institute Of Medicinal Plant Development Chinese Academy Of Medical Sciences And Peking Union Medica
-
Akao Teruaki
Department Of Applied Pharmacology Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceuti
-
Hattori Masao
Metabolic Engineering Institute Of Natural Medicine University Of Toyama
-
Akao Teruaki
Institute Of Natural Medicine Toyama Medical
-
Nakamura Norio
Department Of Biology Yokohama City University
-
Nakamura Norio
Department Of Anatomy Gunma University School Of Medicine
-
Li Yan
Department Of Applied Chemistry Graduate School Of Engineering Kyushu University
-
花岡 美代次
Institute Of Natural Medicine University Of Toyama
関連論文
- Characterization of anti-herpes simplex virus type 1 activity of an alkaloid FK 3000 from Stephania cepharantha
- NT-3 gene modified Schwann cells promote TrkC gene modified mesenchymal stem cells to differentiate into neuron-like cells in vitro
- Gradient decrement of annealing time can improve PCR with fluorescent-labeled primers(METHODS)
- Studies on Antispasmodics. VI. Synthesis of N-Alkyl 6- and 7-Diarylmethyleneindolizidinium Bromides
- Studies on Antispasmodics. V. Synthesis and Anticholinergic Activity of N-Alkyl 1- and 2-Diarylmethylene-indolizidinium Bromides
- 鎮痙剤の研究(第4報)Diarylhydroxymethylquinolizidine Methohalidesの合成と抗アセチルコリン作用
- 鎮痙剤の研究(第3報)2位置換Quinolizidine類の簡便な新合成法とそれらの立体構造について
- 鎮痙剤の研究(第2報)3位置換Quinolizidine類の簡便な新合成法とそれらの立体構造について
- Studies on 1-Azabicyclo Compounds. XXVI. Synthesis of Ten-membered Ring Amines from 9a-Cyanomethyl-, 9a-Ethoxycarbonylmethyl-, and 1-Ethoxycarbonyl-octahydroquinolizine
- Synthesis of (±)-Methyldecamine
- Dacarbazineの光分解によって生成する発痛物質の探索
- Convenient Synthesis of 2,3,9,10-Tetraoxygenated Protoberberine Alkaloids and Their 13-Methyl Alkaloids
- 12-3-B3 ダカルバジン注射剤投与時における血管痛原因物質の探索
- 100(P24) 抗腫瘍性スチリルラクトン化合物の高立体選択的合成(ポスター発表の部)
- (o-トリメチルシリルベンズアルデヒド)クロム錯体を活用する高立体選択的反応の開発
- Erythro-Selective Aldol Reaction of Tricarbonyl(η^6-o-trialkylsilylbenzaldehyde)chromium(0) Complexes with Cyclic Ketene Silyl Acetals
- 18 Protoberberineアルカロイドの新規合成とBenzo[c]phenanthridineアルカロイド(±)-Ambinineの全合成(口頭発表の部)
- Chemical Transformation of Protoberberines. XVII. Biomimetic Introduction of an Oxy Functionality at the C-10 Position in the Benzo[c]phenanthridine Skeleton : Synthesis of 2,3,7,8,10-Pentaoxygenated Benzo[c]phenanthridine Alkaloids, Chelilutine and Sangu
- A FIRST TOTAL SYNTHESIS OF (±)-AMBININE
- Chemical Transformation of Protoberberines. XVI. Regioselective Introduction of an Oxy Functionality at the C_-Position of the Benzo[c]phenanthridine Skeleton : A Convenient Synthesis of Macarpine from Oxychelirubine
- Trimethylsilyl Trifluoromethanesulfonate-Catalyzed Aldol Reaction of Various Aldehydes with Silyl Enol Ethers
- A NOVEL DIASTEREOSELECTIVITY IN THE ALDOL REACTION OF CYCLIC KETENE SILYL ACETALS WITH COBALT-COMPLEXED AND UNCOMPLEXED PROPYNALS
- 学術貢献賞受賞 北 泰行氏の業績
- 故荒田義雄先生を偲んで
- イソキノリンアルカロイドの生合成的合成
- サマリーを重要視(キーワードの使い方)
- 科学研究費の配分について(文部省科学研究費申請と配分の問題点)
- 双環状アミン体から中環状アミン類の合成
- Synthetic Studies on Lythraceae Alkaloids. V. Total Synthesis of (±)-Vertaline
- Synthetic Studies on Lythraceae Alkaloids. IV. Total Synthesis of (±)-Methyldecinine
- Total Synthesis of (±)-Lagerine
- Synthetic Studies on Lythraceae Alkaloids. III. Stereoselective Total Synthesis of (±)-Decaline
- Synthetic Studies on Lythraceae Alkaloids. II. The Mannich Reaction of Isopelletierine with 3-Methoxybenzaldehyde and 3-Hydroxybenzaldehyde
- Studies on 1-Azabicyclo Compounds. XXIV. Synthesis and Stereochemistry of 10-Ethyl-, 10-Vinyl-, and 10-Ethynyl-quinolizidine Methiodides
- Studies on 1-Azabicyclo Compounds. XXII. Stereochemistry of 9a-Substituted Quinolizidine Methiodides
- Metabolism and pharmacokinetics in rats of ganoderiol F, a highly cytotoxic and antitumor triterpene from Ganoderma lucidum
- Pharmacokinetic study of benzoylmesaconine in rats using an enzyme immunoassay system
- Protective Effects of a Neutral Polysaccharide Isolated from the Mycelium of Antrodia cinnamomea on Propionibacterium acnes and Lipopolysaccharide Induced Hepatic Injury in Mice
- Quantitative Determination of Bitter Principles in Specimens of Ganoderma lucidum Using High-Performance Liquid Chromatography and Its Application to the Evaluation of Ganoderma Products
- Chemical Transformation of Protoberberines. XV. : A Novel and Efficient Method for the Introduction of Alkyl Groups on the C-13 Position in the Protoberberine Skeleton
- A NOVEL TOTAL SYNTHESIS OF (±)-CRYPTAUSTOLINE, A DIBENZOPYRROCOLINE ALKALOID
- A NOVEL AND BIOMIMETIC SYNTHESIS OF CHELILUTINE AND SANGUILUTINE
- A TOTAL SYNTHESIS OF (±)-CEPHALOTAXINAMIDE
- 64 ProtoberberineアルカロイドからBenzo [c] phenanthridineアルカロイドの生合成的合成(口頭発表の部)
- 58 ProtoberberineアルカロイドからBenzo[c]phenanthridineアルカロイドの高立体選択的合成
- 33 ProtoberberineからBenzo〔c〕phenanthridine及び3-Arylisoquinolineアルカロイドの合成
- Chemical Transformation of Protoberberines. VIII. A Novel Synthesis of (±)-Fumaricine and a Formal Synthesis of (±)-Alpinigenine
- Chemical Transformation of Protoberberines. VII. Efficient Conversion of Protoberberines into Benzindenoazepines via 8,14-Cycloberbines
- Chemical Transformation of Protoberberines. V. Photochemical Valence Isomerization of Berberinephenolbetaines to 8,14-Cycloberbines, Versatile Aziridine Derivatives for a Novel and Efficient Entry to Spirobenzylisoquinolines and Benzindenoazepines
- Effect of Surface Stress around the S_A Step of Si(001) on the Dimer Structure Determined by Noncontact Atomic Force Microscopy at 5K
- Force Microscopy Imaging of Rest Atom on Si(111)7×7 Surface under Strong Tip-Surface Interaction(Condensed matter: structure and mechanical and thermal properties)
- Visceral hypersensitivity following cold water intake in subjects with irritable bowel syndrome
- Endothelium-dependent vasodilator effect of tannin extract from Cinnamonomi Cortex on isolated rat aorta
- Two New Triterpenes from the Rhizome of Dryopteris crassirhizoma, and Inhibitory Activities of Its Constituents on Human Immunodeficiency Virus-1 Protease
- Enantioselective Dehydroxylation of Enterodiol and Enterolactone Precursors by Human Intestinal Bacteria(Pharmacognosy)
- Developing of anti-HIV agents from natural resources(Chemical & Pharmacological study)
- A New Enzyme Immunoassay for Aconitine and Its Application to Quantitative Determination of Aconitine Levels in Plasma(Pharmacognosy)
- Sesquiterpenes and Alkaloids from Lindera chunii and Their Inhibitory Activities against HIV-1 Integrase
- Flavanone and Flavonol Glycosides from the Leaves of Thevetia peruviana and Their HIV-1 Reverse Transcriptase and HIV-1 Integrase Inhibitory Activities
- Inhibition of Cytopathic Effect of Human Immunodeficiency Virus Type-1 by Various Phorbol Derivatives
- Screening of medicinal plant extracts for estrogenic activity in combination with a glycosidase treatment
- Development of an enzyme immunoassay system for mesaconitine and its application to the disposition study on mesaconitine
- Enzyme immunoassay for the quantitative determination of ganoderic acid A from Ganoderma lucidum
- New Lanostane Triterpene Lactones from the Vietnamese Mushroom Ganoderma colossum (FR.) C. F. BAKER
- Estrogenic and Anti-estrogenic Activities of Cassia tora Phenolic Constituents
- Isolation and Characterization of a Human Intestinal Bacterium, Eubacterium sp. ARC-2, Capable of Demethylating Arctigenin, in the Essential Metabolic Process to Enterolactone(Pharmacology)
- Protective effects of an acidic polysaccharide isolated from fruiting bodies of Ganoderma lucidum against murine hepatic injury induced by Propionibacterium acnes and lipopolysaccharide
- Inhibitory effect of Yunnan traditional medicines on hepatitis C viral polymerase
- In vivo antitumor effects of bitter principles from the antlered form of fruiting bodies of Ganoderma lucidum
- Three New Lignans, Longipedunins A-C, from Kadsura longipedunculata and Their Inhibitory Activity against HIV-1 Protease
- Antiviral Activity of the Marine Alga Symphyocladia latiuscula against Herpes Simplex Virus (HSV-1) in Vitro and Its Therapeutic Efficacy against HSV-1 Infection in Mice(Pharmacognosy)
- Two Proteins, Mn^, and Low Molecular Cofactor Are Required for C-Glucosyl-Cleavage of Mangiferin(Biochemistry)
- Isolation of a Human Intestinal Bacterium That Transforms Mangiferin to Norathyriol and Inducibility of the Enzyme That Cleaves a C-Glucosyl Bond(Pharmacognosy)
- Constituents of the seeds of Glycyrrhiza uralensis
- Chemical and Biological Evaluation of Germinated and Mature Antler-shaped Fruiting Bodies of Ganoderma lucidum
- Protective effect of bicyclol on liver injury induced by hepatic warm ischemia/reperfusion in rats
- Quantitative determination of maleic and succinic acid derivatives in the mycelium of Antrodia cinnamomea
- Conversion of aconitine lipoaconitine by human intestinal bacteria and their antinociceptive effects in mice
- Transformation of Arctiin to Estrogenic and Antiestrogenic Substances by Human Intestinal Bacteria
- Association of Ala589Ser polymorphism of WNK4 gene with essential hypertension in a high-risk Chinese population
- Anti-herpes simplex virus activities of traditional Chinese medicines, used in Yunnan and Tibetan provinces of China
- Structure and Deposition Mechanism of 10-Undecenoic Acid Self-Assembled Layers on H-Si(111) Surfaces Studied by Atomic Force Microscopy and Fourier-Transform Infrared
- Cardiac Functional Analysis by Electrocardiography, Echocardiography and in situ Hemodynamics in Streptozotocin-Induced Diabetic Mice
- New Triterpene Aldehydes, Lucialdehydes A-C, from Ganoderma lucidum and Their Cytotoxicity against Murine and Human Tumor Cells
- Inhibitory effect of Tibetan medicinal plants on viral polymerases
- Inhibitory Effects of Triterpene-Azidothymidine Conjugates on Proliferation of Human Immunodeficiency Virus Type 1 and Its Protease
- Biotransformation of Phorbol by Human Intestinal Bacteria
- Endothelin signaling axis activates osteopontin expression through PI3 kinase pathway in A375 melanoma cells
- Expression of GLUT-1 in psoriasis and the relationship between GLUT-1 upregulation induced by hypoxia and proliferation of keratinocyte growth
- Estrogenic and Antiestrogenic Activities of the Roots of Moghania philippinensis and Their Constituents (Pharmacognosy)
- Generation of Debris in Water-Assisted Femtosecond Laser Drilling of Silica Glass
- Characterization of Micro-Channels Fabricated by In-Water Ablation of Femtosecond Laser Pulses
- Cardioprotective Effect of Orally Administered Angiotensin-Converting Enzyme Inhibitor Against Ischemia : Reperfusion Injury in the Isolated Rat Heart
- Breast-feeding in Bangkok, Thailand: Current status, maternal knowledge, attitude and social support
- Prevalence and correlates of malnutrition among children in rural minority areas of China
- Surface Plasmon Resonance Immunosensor for IgE Analysis Using Two Types of Anti-IgE Antibodies with Different Active Recognition Sites
- Nicorandil Pretreatment and Improved Myocardial Protection During Cold Blood Cardioplegia
- Quantitative determination of apigenin and its metabolism in rat plasma after intravenous bolus administration by HPLC coupled with tandem mass spectrometry
- Jet ejection from droplets near the Leidenfrost temperature
- An experimental investigation of flow-induced acoustic resonance and flow field in a closed side branch system using a high time-resolved PIV technique