In Vitro Maintenance of Liver Function in Hierarchical Co-Culture of Hepatocytes and Non-Parenchymal Liver Cells
スポンサーリンク
概要
- 論文の詳細を見る
Hepatocytes and non-parenchymal liver cells were isolated from adult rat liver and co-cultured in a monolayer. Induction of tyrosine aminotransferase (TAT), which is a hepatocyte-specific function, was enhanced by the non-parenchymal cells. A soluble factor was concluded to be involved in the enhancement because a conditioned medium prepared from non-parenchymal cells was also stimulatory. A hierarchical co-culture, in which hepatocytes and non-parenchymal cells were separated by a collagen layer and which was designed to mimic the in vivo microenvironment of the liver for the long-term maintenance of liver functions was carried out. In this configuration, the level of TAT induction was significantly higher than in the monolayer co-culture. The soluble factor could diffuse through the collagen layer without dispersing into the medium to effectively reach and stimulate hepatocytes. The stimulatory effect was dependent on both the concentration of collagen and the number of non-parenchymal cells. Among the non-parenchymal cells, Kupffer cells were confirmed to be responsible for the enhancement. When cultured alone in a monolayer, hepatocytes completely lost their TAT induction ability within three days, whereas hepatocytes co-cultured with non-parenchymal cells, both in a monolayer and hierarchically, continued to maintain this ability well beyond three days. In particular, a hierarchical co-culture significantly extended the longevity of the hepatocyte function. Effectiveness of the hierarchical co-culture was also shown in the ability of urea synthesis by hepatocytes. The employement of non-parenchymal cells together with hepatocytes in a hierarchical configuration is thus considered to be a promising means of constructing an effective bioartificial liver support.
- 社団法人日本生物工学会の論文
- 1995-12-25
著者
-
近藤 昌夫
Graduate School Of Pharmaceutical Sciences Osaka University:department Of Pharmaceutics And Biopharm
-
Yagi K
Graduate School Of Pharmaceutical Sciences Osaka University
-
Yagi K
Osaka Univ. Osaka Jpn
-
Kawase M
Osaka Univ. Osaka Jpn
-
Kawase Masaya
Faculty of Pharmaceutical Sciences, Osaka-ohtani University
-
Kawase Masaya
Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot
-
NAKASHIMA Yumiko
Graduate School of Pharmaceutical Sciences, Osaka University
-
YAGI Kiyohito
Faculty of Pharmaceutical Sciences, Osaka University
-
MICHIBAYASHI Nobuyasu
Faculty of Pharmaceutical Sciences, Osaka University
-
NAKASHIMA Yumiko
Faculty of Pharmaceutical Sciences, Osaka University
-
MIZOGUCHI Tadashi
Faculty of Pharmaceutical Sciences, Osaka University
-
Yagi Kiyohito
Department Of Biocehmical Engineering Faculty Of Pharmaceutical Sciences Osaka University
-
Mizoguchi T
Gakushuin Univ. Tokyo Jpn
-
Mizoguchi T
Laboratory Of Bio-functional Molecular Chemistry Graduate School Of Pharmaceutical Sciences Osaka Un
-
Mizoguchi Tadashi
Graduate School Of Pharmaceutical Sciences Osaka University
-
Kawase Mitsuo
Ngk Insulators Ltd.
-
Kawase M
Research And Development Center Kobayashi Pharmaceutical Co. Ltd.
-
MIURA YOSHIHARU
Faculty of Pharmaceutical Sciences, Osaka University
-
MIURA YOSHIHARU
Kansai Electric Power Co., Technical Research Center, Environmental Research Center
-
SUMIYOSHI NOBUAKI
Faculty of Pharmaceutical Sciences, Osaka University
-
YAMADA CHIKAOMI
Faculty of Pharmaceutical Sciences, Osaka University
-
Kawase Masaya
Faculty Of Education Kagawa University
-
Yamada Chikaomi
Faculty Of Pharmaceutical Sciences Osaka University
-
Miura Y
Kansai Electric Power Co. Technical Research Center Environmental Research Center
-
Michibayashi Nobuyasu
Faculty Of Pharmaceutical Sciences Osaka University
-
Nakashima Yumiko
Graduate School Of Pharmaceutical Sciences Osaka University
-
Mizoguchi Tadaaki
Research Center Of Supercritical Fluid Technology/research Institute For Environmental Conservation
-
Sumiyoshi Nobuaki
Faculty Of Pharmaceutical Sciences Osaka University
-
Mizoguchi Tadashi
Faculty Of Pharmaceutical Sciences Osaka University
-
Kawase Masaya
Faculty Of Bioscience Nagahama Institute Of Bio-science And Technology
関連論文
- Structure-activity relationships of anthraquinones on the suppression of DNA-binding activity of the aryl hydrocarbon receptor induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin(BIOCHEMICAL ENGINEERING)
- Morphological regulation and aggregate formation of rabbit chondrocytes on dendrimer-immobilized surfaces with D-glucose display(CELL AND TISSUE ENGINEERING)
- Glucose Transporter Mediation Responsible for Morphological Changes of Human Epithelial Cells on Glucose-Displayed Surfaces(CELL AND TISSUE ENGINEERING)
- Synergistic Effect of D-Glucose and Epidermal Growth Factor Display on Dynamic Behaviors of Human Epithelial Cells(CELL AND TISSUE ENGINEERING)
- Response of Human Epithelial Cells to Culture Surfaces with Varied Roughnesses Prepared by Immobilizing Dendrimers with/without D-Glucose Display(CELL AND TISSUE ENGINEERING)
- Availability of Bone Marrow Stromal Cells in Three-Dimensional Coculture with Hepatocytes and Transplantation into Liver-Damaged Mice(MEDICAL BIOTECHNOLOGY, Advances in Biomedical Science and Engineering)
- Maintenance of Hepatocyte Functions by Coculture with Bone Marrow Stromal Cells (MEDICAL BIOTECHNOLOGY)
- Dendrimer-Immobilized Culture Surface as a Tool to Evaluate Formation of Cellular Cytoskeleton of Anchorage-Dependent Cells (BIOCHEMICAL ENGINEERING)
- タイトジャンクション構成蛋白質Claudinを利用した新規薬物送達方法の構築
- Effect of Rapessed and Dietary Oils on the Mean Survival Time of Stroke-Prone Spontaneously Hypertensive Rats
- Effects of Dietary Vegetable Oils on Behavior and Drug Responses in Mice
- Hepatoprotective Effect of Vitamin B_ on Dimethylnitrosamine-Induced Liver Injury(Pharmacology)
- Protection against D-Galactosamine-Induced Acute Liver Injury by Oral Administration of Extracts from Lentinus edodes Mycelia(Pharmacology)
- Suppression of Cell Death in Primary Rat Hepatocytes by α_1-Acid Glycoprotein(Cell and Tissue Engineering)
- Novel Function of Rare Catechin, Epigallocatechin-3-(3"-O-methyl)gallate, against Cold Injury in Primary Rat Hepatocytes(MISCELLANEOUS)
- Transplantation of Hepatocytes Cultured on Hydroxyapatite into Nagase Analbuminemia Rats (MEDICAL BIOTECHNOLOGY)
- Enhancing Effect of Zinc on Hepatoprotectivity of Epigallocatechin Gallate in Isolated Rat Hepatocytes
- Antioxidative Activity of (-)-Epigallocatechin-3-(3"-O-methyl) gallate Isolated from Fresh Tea Leaf and Preliminary Results on Its Biological Activity
- Effectiveness of Polyamidoamine Dendrimers Modified with Tripeptide Growth Factor, Glycyl-L-Histidyl-L-Lysine, for Enhancement of Function of Hepatoma Cells
- Immobilization of Tripeptide Growth Factor Glycyl-L-histidyl-L-lysine on Poly(vinylalcohol)-quarternized Stilbazole (PVA-SbQ) and Its Use as a Ligand for Hepatocyte Attachment
- Cytostatic effect of phenobarbital on a human hepatoma cell line
- Note of assay of hepatic P-450 activity for evaluation of the function of bioartificial liver support system
- Oxidation of 2-Methoxyphenol by Synthetic Copper-ferrite
- Effectiveness of Fructose-Modified Chitosan as a Scaffold for Hepatocyte Attachment
- Effect of zinc on hepatoprotectivity of α1-acid glycoprotein
- Hepatoprotective Effect of Extracts from Lentinus edodes Mycelia on Dimethylnitrosamine-Induced Liver Injury(Pharmacology)
- Liver Protection by Bis (Maltolato) Zinc(II) Complex
- In Vivo Cloning of a Pseudomonas oxalaticus σ^-Like Gene Acting in Expression of Hydrogenase Genes From Alcaligenes hydrogenophilus
- Alcaligenes hydrogenophilus Lithoautotrophy Mutants Defective in Hydrogenase(s) Encoded on Plasmid and σ^-Like Factor Encoded on Chromosome
- In Vivo Cloning of Genes Determining Lithoautotrophy(Aut)on a Plasmid from Alcaligenes hydrogenophilus
- Protective Effect of Montmorillonite on Plasmid DNA in Oral Gene Delivery into Small Intestine(Miscellaneous)
- Polyethyleneimine/Chitosan Hexamer-Mediated Gene Transfection into Intestinal Epithelial Cell Cultured in Serum-Containing Medium(MEDICAL BIOTECHNOLOGY)
- Mechanism of Enhancement Effect of Dendrimer on Transdermal Drug Permeation through Polyhydroxyalkanoate Matrix(MISCELLANEOUS)
- Novel Transdermal Drug Delivery System with Polyhydroxyalkanoate and Starburst Polyamidoamine Dendrimer
- Terahertz Absorption Spectra of Original and Generic Ceftazidime
- Growth Inhibition of Human Leukemia HL-60 Cells by an Antisense Phosphodiester Oligonucleotide Encapsulated into Fusogenic Liposomes
- Characterization of Recombinant Cosmids Containing H_2-Oxidation Genes from Alcaligenes hydrogenophilus
- Transmissibility of Hydrogen Oxidation (Hox) Plasmid from Alcaligenes hydrogenophilus
- Increase in Thioredoxin Activity of Intestinal Epihelial Cells Mediated by Oxidative Stress
- Excretion of Glycerol by the Marine Chlamydomonas sp.Strain W-80 in High CO_2 Cultures
- Application of Glutaraldehyde-Crosslinked Chitosan as a Scaffold for Hepatocyte Attachment
- Acceleration of Starch Degradation by Suppression of H_2 Evolution in Chlamydomonas sp. MGA161
- In Vitro Maintenance of Liver Function in Hierarchical Co-Culture of Hepatocytes and Non-Parenchymal Liver Cells
- Effect of Polyaniline-coated Surface on the Formation of Multicellular Spheroids of Hepatocytes
- In Vivo Recombination of Two R68.45-Primes, One Carrying Plasmid Genes for Autotrophy (Aut) from Alcaligenes hydrogenophilus and the Other Carrying a σ^-Like Chromosome Gene from Pseudomonas oxalaticus
- Enhancement of Starch Degradation by CO_2 in a Marine Green Alga, Chlamydomonas sp. MGA161
- New ent-Kaurene-Type Diterpenoids Possessing Cytotoxicity from the New Zealand Liverwort Jungermannia Species
- A Novel Strategy for a Drug Delivery System Using a Claudin Modulator
- Efficient Gene Transfer into Differentiated Human Trophoblast Cells with Adenovirus Vector Containing RGD Motif in the Fiber Protein(Biopharmacy)
- Evaluation of the Compaction Properties of a Solid Dispersion of Indomethacin with Crospovidone by Tableting Process Analyzer
- Efficient Gene Transfer into Human Trophoblast Cells with Adenovirus Vector Containing Chimeric Type 5 and 35 Fiber Protein(Biopharmacy)
- A Novel Method for Predicting Disintegration Time in the Mouth of Rapidly Disintegrating Tablet by Compaction Analysis Using TabAll(Communication to the Editor)
- Cytotoxic and Apoptosis-Inducing ent-Kaurane-Type Diterpenoids from the Japanese Liverwort Jungermannia truncata NEES
- Supplementary Treatment of Atopic Dermatitis Patients by Choosing Foods to Lower the N-6/N-3 Ratio of Fatty Acids
- Mossbauer Studies on Particle Volume Distribution of α-FeOOH in Rust Formed on Weathering Steel
- Oxidation and Disproportionation of Wustite Studied by Mossbauer Spectroscopy
- Structural Change of Amorphous Mg_Zn_ Alloy under Isothermal Annealing
- Low-Temperature Electron Spin Susceptibility in YBa_2Cu_3O_
- Magnetic Susceptibility of YBa_2Cu_3O_ with Various Oxygen Deficiency
- Dimensionality of the Superconductivity in YBa_2Cu_3O_
- Antiferromagnetic Transition in Y_2BaCuO_5
- Screening of Bacteriophages Producing Endo-N-Acetylneuraminidase
- Optical Properties and Growth Process of Thin Copper Films on Glass Substrate Studied by In-Situ Ellipsometry
- A High Linoleate and a High α-Linolenate Diet Induced Changes in Learning Behavior of Rats. Effects of a Shift in Diets and Reversal of Training Stimuli
- Molecular Cloning and Characterization of a Novel Bacteriophage-Associated Sialidase
- Electrons and Positrons in Amorphous Mg_Zn_
- Note in Statistical Treatment of Medical and Pharmaceutical Data(METHODS, Advances in Biomedical Science and Engineering)
- Application of Partial Least Square on Quantitative Analysis of L-, D-, and DL-Tartaric Acid by Terahertz Absorption Spectra
- CHARACTERIZATON OF LENS GLUTATHIONE S-TRANSFERASES
- 3-Keto Reductase Activity of Estradiol-17β Dehydrogenase and Its Contribution to Androgen Metabolism
- HUMAN THIOLTRANSFERASE : ITS PURIFICATION AND PHYSIOLOGICAL ROLE
- Chemical Recycling of Polycarbonate to Raw Materials by Thermal Decomposition with Calcium Hydroxide/Steam
- High Selective Conversion of Poly(ethylene terephthalate) into Oil Using Ca(OH)_2
- Spectroscopic Ellipsometry of Thin Copper Films on Glass Substrates
- D-Erythrulose Reductase Can Also Reduce Diacetyl: Further Purification and Characterization of D-Erythrulose Reductase from Chicken Liver
- Hepatoprotective Effect of Syringic Acid and Vanillic Acid on CCl4-Induced Liver Injury
- Effect of Chemical Modification of Tyrosine Residues on Activities of Bacterial Lipase
- Improvement of Heat Stability of Yeast Lipase by Chemical Modification with a Heterobifunctional Photogenerated Reagent
- Application of Terahertz Absorption Spectroscopy to Evaluation of Aging Variation of Medicine
- Stably Sustained Hydrogen Production with High Molar Yield through a Combination of a Marine Green Alga and a Photosynthetic Bacterium
- Hepatoprotective Effect of Syringic Acid and Vanillic Acid on Concanavalin A-Induced Liver Injury
- Mitochondrial Dynamics of Yeast during Sake Brewing(BREWING AND FOOD TECHNOLOGY)
- Effects of Non-covalent Interaction of Dimethyl Suberimidate on Lipase Stability(Biological Chemistry)
- Chlorine Occupancy Dependence of Crystal Structure of Pure β-Phase of Iron-Oxyhydroxide
- Hard Magnetic Properties of Nd-Fe-B Alloys Crystallized from Amorphous Phase : Magnetism, Magnetic Materials and Devices
- Origin of Magnetic Contrast in Spin-Polarized Scanning Tunneling Spectroscopy: Experiments on Ultra-Thin Mn Films
- Small Angle X-Ray Reflection and Conductivity of Ni Films Prepared by Jon Beam Sputtering and Electron Beam Deposition
- Free-Volume-Dependent Atomic Diffusion in Compositionally Modulated Amorphous Co-Zr Films
- FERRIMAGNETISM IN AMORPHOUS ALLOYS
- BASIC MAGNETIC PROPERTIES OF AMORPHOUS ALLOYS
- New Structure Descriptor in the Structure-Activity Relationship Study on the Suppression of Aryl Hydrocarbon Receptor Transformation by Anthraquinones
- Effect of Green Tea Extract on Copper Dynamics in Mouse Hair
- Effect of Green Tea Extract on Copper Dynamics in Mouse Hair