Maintenance of Hepatocyte Functions by Coculture with Bone Marrow Stromal Cells (MEDICAL BIOTECHNOLOGY)
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概要
- 論文の詳細を見る
Rat hepatocytes and bone marrow stromal cells (BMSCs) were cocultured with the aim of maintaining differentiated hepatocyte functions. After BMSCs were expanded to a confluent monolayer, freshly isolated hepatocytes were cultured with them, separated by a semipermeable membrane. The BMSCs significantly increased the urea synthesis and albumin secretion activities of the hepatocytes. Conditioned medium prepared from a BMSC monoculture had the same effect. Further study showed that interleukin-6 was involved in the maintenance of urea synthesis and another factor in the maintenance of albumin secretion.
- 社団法人日本生物工学会の論文
- 2004-05-25
著者
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近藤 昌夫
Graduate School Of Pharmaceutical Sciences Osaka University:department Of Pharmaceutics And Biopharm
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Kawase Masaya
Graduate School Of Pharmaceutical Sciences Osaka Univ.
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近藤 昌夫
Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Kawase Masaya
Nagahama Institute of Bio-science and Technology
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Yagi Kiyohito
Graduate School of Pharmaceutical Sciences, Osaka University
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Yagi K
Graduate School Of Pharmaceutical Sciences Osaka University
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Yagi Kiyohito
Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Yagi K
Osaka Univ. Osaka Jpn
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Kawase M
Nagahama Institute Of Bio-science And Technology
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Kawase M
Osaka Univ. Osaka Jpn
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Taya Masahito
Division Of Chemical Engineering Graduate School Of Engineering Science Osaka University
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Higashiyama Shinji
Graduate School Of Pharmaceutical Sciences Osaka University
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Takeda Masashi
Graduate School of Pharmaceutical Sciences, Osaka University
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Isoda Katsuhiro
Graduate School of Pharmaceutical Sciences, Osaka University
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KOJIMA MIDORI
Graduate School of Pharmaceutical Sciences, Osaka University
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Kawase Masaya
Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot
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Yagi Kiyohito
Department Of Biocehmical Engineering Faculty Of Pharmaceutical Sciences Osaka University
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Kawase Mitsuo
Ngk Insulators Ltd.
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Kawase M
Research And Development Center Kobayashi Pharmaceutical Co. Ltd.
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Kawase Masaya
Faculty Of Education Kagawa University
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Kojima Midori
Graduate School Of Pharmaceutical Sciences Osaka University
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