In Vivo Cloning of Genes Determining Lithoautotrophy(Aut)on a Plasmid from Alcaligenes hydrogenophilus

概要

論文の詳細を見る
Hydrogenase(hox)genes on the megaplasmid pHG21-a from Alcaligenes hydrogenophilus, whose lithoautotrophic growth(Aut)is supported by H_2-oxidation(Hox)and CO_2-fixation(Cfx), were cloned in vivo using a broad host range IncP1 plasmid R68.45. The recombinant plasmid was detected by the characteristic that it was transferred at a frequency 10^6-fold higher than pHG21-a in intrastrain mating of the Hox^- Cfx^+ bacterium Pseudomonas oxalaticus OX1. All of six recombinant plasmids designated pFUs inherited all three resistance markers of R68.45. Four plasmids(pFU3,pFU8,oFU11,and pFU15)with a molecular size of 69 Md had only membrane-bound hydrogenase(hoxP) genes, and two plasmids(pFU7 and pFU9)of 85 Md had both hoxP and soluble hydrogenase(hoxS)genes. The Hox^- Cfx^- bacteria P. oxalaticus OX4 and OX6 gained Aut^+ phenotype by the possession of pHG21-a, pFU7 or pFU15. These results showed that Hox plasmid pHG21-a was an Aut plasmid and pFU7 and pFU15 inherited this phenotype. pFU7 was maintained stably in P. oxalaticus OX1 and had all of the lithoautotrophic phenotypes of pHG21-a. pFU7,rather than pHG21-a, is useful for further studies on the transfer of the Aut phenotype to a broad range of bacteria.
社団法人日本生物工学会の論文
1991-06-25