Regeneration of NADPH by Frozen-thawed Cells of a Blue-green Alga, Synechococcus sp.
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概要
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The photoreduction of NADP+ and its associated reactions were studied in a blue-green algal preparation that was frozen in liquid nitrogen and thawed at room temperature. The preparation was capable of photoreducing exogeneous NADP+. Water was the ultimate electron donor for the reduction. The optimum pH was 7.5-8.0, and optimum temperature, around 50°C. Light saturation for NADP+ photoreduction was reached at 50 μEinstein/m2/sec. Factors limiting the stability of the preparation were examined and a possible application of this cofactor regeneration system is discussed.
- 社団法人 日本農芸化学会の論文
著者
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Yagi Kiyohito
Department Of Bio-functional Molecular Chemistry Graduate School Of Pharmaceutical Sciences Osaka Un
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Miyamoto Kazuhisa
Department Of Biochemical Engineering Faculty Of Pharmaceutical Sciences Osaka University
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Miura Yoshiharu
Department Of Biochemical Engineering Faculty Of Pharmaceutical Sciences Osaka University
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Nakano Yoshihisa
Department Of Agricultural Chemistry
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YAGI KIYOHITO
Department of Biochemical Engineering, Faculty of Pharmaceutical Sciences, Osaka University
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