Dark-Period Nitrogen Fixation by Mastigocladus laminosus and Its Application to a Biophotolysis System

概要

論文の詳細を見る
Nitrogen supply to N-starved cells of Mastigocladus laminosus was investigated to repair cellular components and stabilize their long-term H_2 production. This alga exhibited C_2H_2 reducing activity even in the dark, especially in the presence of added O_2. This O_2-dependent activity was much higher in N-starved cells than in air-grown cells. Thus, when incubated with air in the dark periods of a light/dark cycle, N-starved cultures of M. laminosus fixed an appreciable amount of nitrogen and regreened. Nitrogenase activity in air-incubated cells declined rapidly in the early stage of the dark period, most likely because of repression of nitrogenase synthesis by ammonia or another product of N_2 fixation. In the subsequent light period, however, nitrogenase activities, with respect to both acetylene reduction and H_2 evolution, were quickly restored after short lag periods. Employing this method of nitrogen supply, prolonged H_2 production was demonstrable in an alternating cycle of 16 h light and 8 h dark.
社団法人日本生物工学会の論文
1984-02-25