Inactivation of Hexokinase and Glucose-6-phosphate Dehydrogenase during Immobilization by Polyacrylamid Gel Entrapping
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概要
- 論文の詳細を見る
Hexokinase and glucose-6-phosphate dehydrogenase (G-6-PDH) from yeast were immobilized by polyacrylamide gel entrapping, and activity yields for these enzymes were compared with those for glucoamylase, glucose oxidase, and glucosephosphate isomerase immobilized by the same procedure Significantly low yields were observed for hexokinase and G-6-PDH. Catalysts for the polymerization of acrylamide, ammonium persulfate, and β-dimethylaminopropionitrile, scarcely inactivated these enzymes but incubation with acrylamide monomer at pH 7.8 resuted in significant decrease in activity. Hexokinase and G-6-PDH in acrylamide solution were stabilized by the addition of glucose and NADP^+, respectively. Substrate addition at the time of polymerization resulted in a 2-fold increase in activity yield for both enzymes. The yield for yeast G-6-PDH increased when G-6-PDH modified with 5,5'-dithiobis-(2-nitrobenzoic acid) was immobilized and then treated with excess thiols. It was concluded that modification of the essential sulfhydryl group of yeast G-6-PDH by acylamide monomer was involved in the inactivation during the immobilization. In-activation mechanisms during polyacrylamide gel entrapping are discussed.
- 社団法人日本生物工学会の論文
- 1977-01-25
著者
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MIYAMOTO Kazuhisa
Department of Environmental Bioengineering Laboratory, Graduate School of Pharmaceutical Sciences, O
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MIURA YOSHIHARU
Department of Biochemical Engineering, Faculty of Pharmaceutical Science, Osaka University
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OKAZAKI MITSUO
Department of Applied Biology, Shinshu University
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Miyamoto Kazuhisa
Department Of Biochemical Engineering Faculty Of Pharmaceutical Sciences Osaka University
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Miura Yoshiharu
Department Of Biochemical Engineering Faculty Of Pharmaceutical Sciences Osaka University
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Miura Yoshiharu
Departmento Of Biochemical Engineering Faculty Of Pharmaceutical Sciences Osaka Univerisity
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Okazaki Mitsuo
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Okazaki Mitsuo
Department Of Biochemical Engineering Faculty Of Pharmaceutical Sciences Osaka University
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OHYABU MASAKO
Department of Biochemical Engineering, Faculty of Pharmaceutical Sciences, Osaka Univerisity
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Ohyabu Masako
Department Of Biochemical Engineering Faculty Of Pharmaceutical Sciences Osaka Univerisity
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OKAZAKI MITSUO
Department of Biochemical Engineering, Faculty of Pharmaceutical Sciences, Osaka University
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