Gene Cloning of Cellobiohydrolase II from the White Rot Fungus Irpex lacteus MC-2 and Its Expression in Pichia pastoris

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概要

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• A gene (cel4) coding for a cellobiohydrolase II (Ex-4) was isolated from the white rot basidiomycete, Irpex lacteus strain MC-2. The cel4 ORF was composed of 452 amino acid residues and was interrupted by eight introns. Its deduced amino acid sequence revealed a multi domain structure composed of a cellulose-binding domain, a linker, and a catalytic domain belonging to family 6 of glycosyl hydrolases, from the N-terminus. cel4 cDNA was successfully expressed in the yeast Pichia pastoris. Recombinant Ex-4 showed endo-processive degrading activity towards cellulosic substrates, and a synergistic effect in the degradation of Avicel was observed when the enzyme acted together with either cellobiohydrolase I (Ex-1) or endoglucanase (En-1) produced by I. lacteus MC-2.

• 社団法人 日本農芸化学会の論文
• 2008-12-23

著者

• Toda Hiroshi

  Department Of Surgery Seirei Sumiyoshi Hospital

• NOZAKI Kouichi

  Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

• OKAZAKI MITSUO

  Department of Applied Biology, Shinshu University

• SHIMOSAKA MAKOTO

  Department of Applied Biology, Shinshu University

• Shiroishi Masahiro

  Agricultural Technology Institute Of Nagano Farmers' Federation

• Shiroishi Masahiro

  Department Of Physics Graduate School Science University Of Tokyo

• Shiroishi Masahiro

  Department Of Physics Graduate School Of Science University Of Tokyo

• Nozaki Kouichi

  Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University

• KANDA Takahisa

  Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University

• AMANO Yoshihiko

  Department of Chemistry and Material Engineering, Faculty of Engineering, Shinshu University

• Kanda Takahisa

  Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University

• Amano Yoshihiko

  Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University

• Shimosaka Makoto

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Okazaki M

  Dep. Of Applied Biology Fac. Of Textile Sci. And Technol. Shinshu Univ.

• Okazaki Mitsuo

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• 城石 正弘

  Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Toda Hiroshi

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Toda Hiroshi

  Department Of Anesthesia Kyoto University Hospital

• NAGAHATA Naoki

  Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University

• Nishizawa Kenichi

  Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University

• Arikawa Y

  Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University

• Nagahata Naoki

  Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University

• Kuroyanagi Tomoko

  Faculty Of Textile Science And Technology Shinshu University

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