Cloning, Expression, and Characterization of a Chitinase from the Chitinolytic Bacterium Aeromonas hydrophila Strain SUWA-9
スポンサーリンク
概要
- 論文の詳細を見る
The chitinolytic bacterium Aeromonas hydrophila strain SUWA-9, which was isolated from freshwater in Lake Suwa (Nagano Prefecture, Japan), produced several kinds of chitin-degrading enzymes. A gene coding for an endo-type chitinase (chiA) was isolated from SUWA-9. The chiA ORF encodes a polypeptide of 865 amino acid residues with a molecular mass of 91.6 kDa. The deduced amino acid sequence showed high similarity to those of bacterial chitinases classified into family 18 of glycosyl hydrolases. chiA was expressed in Escherichia coli and the recombinant chitinase (ChiA) was purified and examined. The enzyme hydrolyzed N-acetylchitooligomers from trimer to pentamer and produced monomer and dimer as a final product. It also reacted toward colloidal chitin and chitosan with a low degree of deacetylation. When cells of SUWA-9 were grown in the presence of colloidal chitin, a 60 kDa-truncated form of ChiA that had lost the C-terminal chitin-binding domain was secreted.
- 社団法人 日本農芸化学会の論文
- 2006-10-23
著者
-
ZHANG Xin
Department of Anatomy, Chonbuk National University Medical School
-
Zhang Xin
Department Of Anatomy Chonbuk National University Medical School:research Center For Allergic Immune
-
SHIMOSAKA MAKOTO
Department of Applied Biology, Shinshu University
-
Shimosaka M
信州大・繊維・応生系
-
Shimosaka Makoto
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
-
LAN Xiqian
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
-
HU Junhua
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
-
Hu Junhua
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
-
Lan Xiqian
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
-
Zhang Xin
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
関連論文
- Analysis of a change in bacterial community in different environments with addition of chitin or chitosan(ENVIRONMENTAL BIOTECHNOLOGY)
- Sex difference in cellular proliferation within the telencephalic ventricle zone of Bengalese finch
- Inhibitory Effects of Agaricus blazei on Mast Cell-Mediated Anaphylaxis-Like Reactions(Pharmacology)
- Inhibition of Anaphylaxis-Like Reaction and Mast Cell Activation by Water Extract from the Fruiting Body of Phellinus linteus(Pharmacology)
- An Evaluation of the Biological Effects of Three Different Modes of Magnetic Fields on Cultured Mammalian Cells
- Analysis of a Promoter-Like Region in Flavobacterium Which Controls 6-Aminohexanoic Acid Linear Oligomer Hydrolase Gene Expression in Escherichia coli
- Isolation and characterization of a gene coding for chitin deacetylase specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes and its expression in the yeast Pichia pastoris
- Isolation and analysis of genes specif ically expressed during fruiting body development in the basidiomycete Flammulina velutipes by fluorescence differential display
- Cloning and characterization of a gene coding for a hydrophobin, Fv-hyd1, specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes
- Isolation of Sake Yeast Strains Possessing Various Levels of Succinate- and/or Malate-Producing Abilities by Gene Disruption or Mutation
- Effect of Gene Disruptions of the TCA Cycle on Production of Succinic Acid in Saccharomyces cerevisiae
- Effect of Salt Stress on Plasma Membrane Permeability and Lipid Saturation in the Salt-Tolerant Yeast Zygosaccharomyces rouxii
- Isolation and Characterization of Zygosaccharomyces rouxii Mutants Defective in Proton Pumpout Activity and Salt Tolerance
- Cloning of the SAT1 Gene Concerned with Salt Tolerance of the Yeast Zygosaccharomyces rouxii
- Gene Cloning of an Endoglucanase from the Basidiomycete Irpex lacteus and Its cDNA Expression in Saccharomyces cerevisiae
- Cloning, Expression, and Characterization of a Chitinase from the Chitinolytic Bacterium Aeromonas hydrophila Strain SUWA-9
- Complete Purification of Yeast Glyoxalase I and Its Activation by Ferrous Ions and Polyamines
- Cloning of Genes Enhancing the Resistance of Saccharomyces cerevisiae to Zinc and Cadmium Ions
- 8-Azaguanine Resistant Mutant of the Yeast, Saccharomyces cerevisiae
- Metabolism of α-Ketoaldehydes in Yeasts : Inducible Formation of Methylglyoxal Reductase and Its Relation to Growth Arrest of Saccharomyces cerevisiae
- Phosphorylation of AMP to ATP by Dried Escherichia coli B Cells
- Cloning and analysis of genes coding for chitinolytic enzymes from the novel chitinolytic bacterium Chitiniphilus shinanonensis
- Analysis of Essential Carboxylic Amino Acid Residues for Catalytic Activity of Fungal Chitosanases by Site-Directed Mutagenesis(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Purification, Cloning, and Sequence Analysis of β-N-Acetylglucosaminidase from the Chitinolytic Bacterium Aeromonas hydrophila Strain SUWA-9
- Effect of Administration with the Extract of Gymnema sylvestre R. Br Leaves on Lipid Metabolism in Rats
- Effect of Long Term-Administration with Gymnema sylvestre R.B_R on Plasma and Liver Lipid in Rats
- Role of Cellulose-Binding Domain of Exocellulase I from White Rot Basidiomycete Irpex lacteus
- Cloning and Characterization of a Chitosanase Gene from the Koji Mold Aspergillus oryzae Strain IAM 2660
- The Bacterium Burkholderia gladioli Strain CHB101 Produces Two Different Kinds of Chitinases Belonging to Families 18 and 19 of the Glycosyl Hydrolases
- 再録 Isolation of sake yeast mutants producting a higher level of ethyl caproate and/or iso-amyl acetate
- Isolation of Sake Yeast Mutants Producing a High Level of Ethyl Caproate and/or Isoamyl Acetate
- Purification and Characterization of Chitosanase and Exo-β-D-Glucosaminidase from a Koji Mold, Aspergillus oryzae IAM2660
- Purification and characterization of UDP-glucose: hydroxycoumarin 7-O-glucosyltransferase, with broad substrate specificity from tobacco cultured cells
- Purification, Characterization and Gene Analysis of Exo-Cellulase II(Ex-2)from the White Rot Basidiomycete Irpex lacteus
- Isolation and Transcriptional Analysis of a Cellulase Gene(cel1) from the Basidiomycete Irpex lacteus
- A Transient Increase of Phenylalanine Ammonia-lyase Transcript in Kinetin-treated Tobacco Callus
- Analysis of the Influence of the Electrical Asynchrony on Regional Mechanics of the Infarcted Left Ventricle Using Electromechanical Heart Models
- Construction and Analysis of a Bacterial Community Exhibiting Strong Chitinolytic Activity
- Characterization of a Gene Conferring Red Fluorescence Isolated from an Environmental DNA Library Constructed from Soil Bacteria
- Characterization of a Gene Conferring Red Fluorescence Isolated from an Environmental DNA Library Constructed from Soil Bacteria
- Gene Cloning of Cellobiohydrolase II from the White Rot Fungus Irpex lacteus MC-2 and Its Expression in Pichia pastoris
- Gene Cloning, Expression, and Characterization of a Second β-N-Acetylglucosaminidase from the Chitinolytic Bacterium Aeromonas hydrophila Strain SUWA-9
- Effect of gene disruptions of the TCA cycle on production of succinic acid in Saccharomyces cerevisiae
- COUMARIN UP-TAKE SYSTEM IN TOBACCO CULTURED CELL
- INDUCTION AND GENETIC ANALYSIS OF PHENYLALANINE AMMONIA-LYASE IN TOBACCO COULTURED CELL
- Cloning and Characterization of Mycovirus Double-stranded RNA from the Plant Pathogenic Fungus, Fusarium solani f. sp. robiniae
- Chitosanase from the Plant Pathogenic Fungus, Fusarium solani f. sp. phaseoli : Purification and Some Properties
- Purification and Some Properties of 2, 4-D-Activated Scooletin Glucosyltransferase from Tobacco Cells
- Transcription of a Gene for 6-Aminohexanoic Acid Linear Oligomer Hydrolase of Flavobacterium sp. KI72 in Escherichia coli
- Cellulose and Xylan Degrading Enzymes of the Plant Pathogenic Fungus, Fusarium oxyspomm SUF850(Microbiology & Fermentation Industry)
- Cell Aggregation and Elongated Cell Morphology Caused by a Mutation Conferring Increased Sensitivity to Indole-3-Acetic Acid in the Yeast : Saccharomyces cerevisiae
- BAFF supports human B cell differentiation in the lymphoid follicles through distinct receptors
- Human leukocyte antigen-G is closely associated with tumor immune escape in gastric cancer by increasing local regulatory T cells
- Cloning and Characterization of a Gene Coding for a Major Extracellular Chitosanase from the Koji Mold Aspergillus oryzae
- Heterologous Expression and Functional Characterization of a Novel Chitinase from the Chitinolytic Bacterium Chitiniphilus shinanonensis
- An Improved Formulation Screening and Optimization Method Applied to the Development of a Self-Microemulsifying Drug Delivery System