Isolation of Sake Yeast Strains Possessing Various Levels of Succinate- and/or Malate-Producing Abilities by Gene Disruption or Mutation
スポンサーリンク
概要
- 論文の詳細を見る
Succinate and malate are the main taste components produced by yeast during sake(Japanese alcohol beverage)fermentation. Sake yeast strains possessing various organic acid productivities were isolated by gene disruption. Sake fermented using the aconitase gene(ACO1)disruptant contained a two-fold higher concentration of malate and a two-fold lower concentration of succinate than that made using the wild-type strain K901. The fumarate reductase gene(OSM1)disruptant produced sake containing a 1.5-fold higher concentration of succinate as compared to the wild-type, whereas the α_ketoglutarate dehydrogenase gene(KGD1)and fumarase gene(FUM1)disruptants gave lower succinate concentrations. The Δkgdl disruptant exhibited lower succinate productivity in the earlier part of the sake fermentation, while the Δfuml disruptant showed lower succinate productivity later in the fermentation, indicating that succinate is mainly produced by an oxidative pathway of the TCA cycle in the early phase of sake fermentation and by a reductive pathway in the later phases. Sake yeasts with low succinate productivity and/or high malate productivity was bred by isolating mutants unable to assimilate glycerol as a carbon source. Low malate-producing yeasts were also obtained from phenyl succinate-resistant mutants. The mutation of one of these mutant strains with low succinate productivity was found to occur in the KGD1 gene. These strains possessing various succinate- and/or malate-producing abilities are promising for the production of sake with distinctive tastes.
- 1999-03-25
著者
-
KOBAYASHI Misato
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
-
ARIKAWA YUKIHIKO
Food Technology Research Institute of Nagano Prefecture
-
OKAZAKI MITSUO
Department of Applied Biology, Shinshu University
-
Shiroishi Masahiro
Department Of Physics Graduate School Science University Of Tokyo
-
Kobayashi M
Research Laboratory Of Higashimaru Shoyu Co. Ltd.
-
Enomoto Koichi
Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University
-
Amano Yoshihiko
Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University
-
SHIMOSAKA Makoto
Gene Research Center, Shinsyu University
-
Shimosaka M
信州大・繊維・応生系
-
Shimosaka Makoto
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
-
Shimosaka Makoto
Gene Research Center Shinsyu University
-
KODAIRA Ritsuko
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
-
MURATSUBAKI HARUHIRO
Section of Clinical Biochemistry, School of Health Science, Kyorin University
-
ENOMOTO KEIICHIRO
Section of Clinical Biochemistry, School of Health Science, Kyorin University
-
Kobayashi Misato
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
-
Okazaki M
Dep. Of Applied Biology Fac. Of Textile Sci. And Technol. Shinshu Univ.
-
Okazaki Mitsuo
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
-
城石 正弘
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
-
Muratsubaki Haruhiro
Section Of Clinical Biochemistry School Of Health Science Kyorin University
-
Arikawa Y
Department Of Chemistry And Material Engineering Faculty Of Engineering Shinshu University
-
Kodaira Ritsuko
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
-
Kobayashi Misato
Department Of Applied Molecular Bioscience Graduate School Of Bioagricultural Sciences Nagoya University
関連論文
- Analysis of a change in bacterial community in different environments with addition of chitin or chitosan(ENVIRONMENTAL BIOTECHNOLOGY)
- 第4回(平成19年度)日本学術振興会賞受賞 : 押川正毅氏(学界ニュース)
- 24pTL-8 スピン1/2XXZ鎖のストリング相関関数の漸近挙動とその応用(量子スピン系,領域11,統計力学,物性基礎論,応用数学,力学,流体物理)
- 18aWA-2 1次元ハバード模型の高次の保存量のハーフフィリングにおける強結合展開(量子スピン系,領域11,原子・分子,量子エレクトロニクス,放射線物理)
- 18aWA-3 Delta=1/2 XXZ鎖のエンタングルメントエントロピー(量子スピン系,領域11,原子・分子,量子エレクトロニクス,放射線物理)
- 25aXD-3 スピン1/2ハイゼンベルクXXZ鎖のストリング相関関数(25aXD 量子スピン系,領域11(統計力学,物性基礎論,応用数学,力学,流体物理))
- 29pUA-2 ハイゼンベルクスピン鎖の基底状態相関関数(29pUA 領域11シンポジウム:可積分量子スピン系の新展開:相関関数と有限温度の厳密解,領域11(統計力学,物性基礎論,応用数学,力学,流体物理))
- 21pXL-6 一次元ハイゼンベルクXXX模型の基底状態の相関関数の厳密計算(量子スピン系,領域11(統計力学,物性基礎論,応用数学,力学,流体物理))
- 19aPS-76 一次元Heisenberg模型の有限温度相関函数の高温展開(領域11ポスターセッション,領域11(統計力学,物性基礎論,応用数学,力学,流体物理))
- Heisenbergスピン鎖の相関関数(最近の研究から)
- 相関関数とゼータ関数 (特集 可解格子模型入門)
- 12aTK-2 XXX 鎖の相関関数の計算(量子可積分系, 領域 11)
- Next Nearest-Neighbor Correlation Functions of the Spin-1/2 XXZ Chain at Massive Region(Condensed Matter : Electronic Structure, Electrical, Magnetic and Optical Properties)
- 31aPS-9 スピン-1/2 XXZ 鎖の Emptiness Formation Probability の漸近的ふるまい
- 28aWE-9 Δ=1/√XXZ 鎖の相関関数
- 量子スピン鎖における新しい相関関数-emptiness formation probability-に関する最近の発展
- 18aRC-4 1次元等方的XY模型のEmptiness Formation Probability
- 18aRC-3 熱力学ベーテ仮説と量子転送行列の等価性
- MHC-LINKED SUSCEPTIBILITY TO TYPE 1 DIABETES IN THE NOD MOUSE : FURTHER LOCALIZATION OF Idd16 BY SUBCONGENIC ANALYSIS
- Production of Recombinant Chicken IgY-Fc and Evaluation of Its Transport Ability into Avian Egg Yolks
- Examination of mitral valve repair with port-access method : Aiming at early and less invasive mitral valve repair
- CONTRIBUTION OF CLASS III MHC TO SUSCEPTIBILITY TO TYPE 1 DIABETES IN THE NOD MOUSE
- Analysis of Sake Mash Using Multichannel Taste Sensor
- Confirmation of Diabetes-Related Quantitative Trait Loci Derived from SM/J and A/J Mice by Using Congenic Strains Fed a High-Carbohydrate or High-Fat Diet
- Analysis of a Promoter-Like Region in Flavobacterium Which Controls 6-Aminohexanoic Acid Linear Oligomer Hydrolase Gene Expression in Escherichia coli
- Involvement of Parathyroid Hormone-Related Peptide in Cell Proliferation Activity of Human Uterine Leiomyomas
- Purification and Properties of an Inulin Fructotransferase (Depolymerizing) from Arthrobacter sp. H65-7
- Production of Inulin Fructotransferase (Depolymerizing) by Arthrobacter sp. H65-7 and Preparation of DFA III from Inulin by the Enzyme
- Fermionic R-Operator and Integrability of the One-Dimensional Hubbard Model
- Fermionic R-Operator for the Fermion Chain Model
- Biological Activities of Abscisic Acid Analogs in the Morphological Change of the Green Alga Haematococcus pluvialis
- Isolation and characterization of a gene coding for chitin deacetylase specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes and its expression in the yeast Pichia pastoris
- Isolation and analysis of genes specif ically expressed during fruiting body development in the basidiomycete Flammulina velutipes by fluorescence differential display
- Cloning and characterization of a gene coding for a hydrophobin, Fv-hyd1, specifically expressed during fruiting body development in the basidiomycete Flammulina velutipes
- Some characteristics of three groups in Flammulina velutipes classified by analysis of esterase isozymes
- Isolation of Sake Yeast Strains Possessing Various Levels of Succinate- and/or Malate-Producing Abilities by Gene Disruption or Mutation
- Effect of Gene Disruptions of the TCA Cycle on Production of Succinic Acid in Saccharomyces cerevisiae
- Morphological Changes in the Life Cycle of the Green Alga Haematococcus pluvialis
- Effect of Salt Stress on Plasma Membrane Permeability and Lipid Saturation in the Salt-Tolerant Yeast Zygosaccharomyces rouxii
- Isolation and Characterization of Zygosaccharomyces rouxii Mutants Defective in Proton Pumpout Activity and Salt Tolerance
- Effect of Carbon/Nitrogen Ratio on Encystment Accompanied with Astaxanthin Formation in a Green Alga, Haematococcus pluvialis
- Large Scale Purification of Recombinant Insulin-Like Growth Factor I (IGF-I, Mecasermin) from a Fused Protein Produced in Escherichia coli
- An Approach to High-Level Production of Mecasermin (Somatomedin C) Fused Protein in Escherichia coli HB101
- Expression of Genes Encoding Membrane-bound Hydrogenase in Pseudomonas hydrogenovora under Autotrophic Condition Is Dependent on Two Different Promoters
- Cloning of the SAT1 Gene Concerned with Salt Tolerance of the Yeast Zygosaccharomyces rouxii
- Gene Cloning of an Endoglucanase from the Basidiomycete Irpex lacteus and Its cDNA Expression in Saccharomyces cerevisiae
- Cloning, Expression, and Characterization of a Chitinase from the Chitinolytic Bacterium Aeromonas hydrophila Strain SUWA-9
- Complete Purification of Yeast Glyoxalase I and Its Activation by Ferrous Ions and Polyamines
- Cloning of Genes Enhancing the Resistance of Saccharomyces cerevisiae to Zinc and Cadmium Ions
- 8-Azaguanine Resistant Mutant of the Yeast, Saccharomyces cerevisiae
- Metabolism of α-Ketoaldehydes in Yeasts : Inducible Formation of Methylglyoxal Reductase and Its Relation to Growth Arrest of Saccharomyces cerevisiae
- Phosphorylation of AMP to ATP by Dried Escherichia coli B Cells
- Cloning and analysis of genes coding for chitinolytic enzymes from the novel chitinolytic bacterium Chitiniphilus shinanonensis
- Analysis of Essential Carboxylic Amino Acid Residues for Catalytic Activity of Fungal Chitosanases by Site-Directed Mutagenesis(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Purification, Cloning, and Sequence Analysis of β-N-Acetylglucosaminidase from the Chitinolytic Bacterium Aeromonas hydrophila Strain SUWA-9
- Effect of Administration with the Extract of Gymnema sylvestre R. Br Leaves on Lipid Metabolism in Rats
- Effect of Long Term-Administration with Gymnema sylvestre R.B_R on Plasma and Liver Lipid in Rats
- Role of Cellulose-Binding Domain of Exocellulase I from White Rot Basidiomycete Irpex lacteus
- Cloning and Characterization of a Chitosanase Gene from the Koji Mold Aspergillus oryzae Strain IAM 2660
- The Bacterium Burkholderia gladioli Strain CHB101 Produces Two Different Kinds of Chitinases Belonging to Families 18 and 19 of the Glycosyl Hydrolases
- 再録 Isolation of sake yeast mutants producting a higher level of ethyl caproate and/or iso-amyl acetate
- Isolation of Sake Yeast Mutants Producing a High Level of Ethyl Caproate and/or Isoamyl Acetate
- Purification and Characterization of Chitosanase and Exo-β-D-Glucosaminidase from a Koji Mold, Aspergillus oryzae IAM2660
- Purification and characterization of UDP-glucose: hydroxycoumarin 7-O-glucosyltransferase, with broad substrate specificity from tobacco cultured cells
- Purification, Characterization and Gene Analysis of Exo-Cellulase II(Ex-2)from the White Rot Basidiomycete Irpex lacteus
- Isolation and Transcriptional Analysis of a Cellulase Gene(cel1) from the Basidiomycete Irpex lacteus
- Synergistic Actions of Exo-Type Cellulases in the Hydrolysis of Cellulose with Different Crystallinities
- Action of Exo- and Endo-Type Cellulases from Irpex lacteus on Valonia Cellulose
- A Transient Increase of Phenylalanine Ammonia-lyase Transcript in Kinetin-treated Tobacco Callus
- Improvement of a Fixed Bed Bioreactor for Plant Cell Culture
- Ascorbic Acid Deficiency Stimulates Hepatic Expression of Inflammatory Chemokine, Cytokine-Induced Neutrophil Chemoattractant-1, in Scurvy-Prone ODS Rats
- Serum Adiponectin Concentration : Its Correlation with Diabetes-Related Traits and Quantitative Trait Loci Analysis in Mouse SMXA Recombinant Inbred Strains
- SMXA-5 Mouse as a Diabetic Model Susceptible to Feeding a High-fat Diet
- Isolation of sake yeast strains possesing various levels of Succinate-and/or malate-producing abilities by gene Distuption or Mutation
- Effects of Light Intensity, Light Quality, and Illumination Cycle on Astaxanthin Formation in a Green Alga, Haematococcus pluvialis
- Growth and Astaxanthin Formation of Haematococcus pluvialis in Heterotrophic and Mixotrophic Conditions
- Astaxanthin Production by a Green Alga, Haematococcus pluvialis Accompanied with Morphological Changes in Acetate Media
- Purification of Staphylococcal Protein A by Affinity Precipitation Using a Reversibly Soluble-Insoluble Polymer with Human IgG as a Ligand
- Soluble fumarate reductase isoenzymes from Saccharomyces cerevisiae are required for anaerobic growth
- Screening and investigation of dye decolorization activities of basidiomycetes
- Mode of Action of Cellulases on Dyed Cotton with a Reactive Dye
- Fine substrate Specificities of Four Exo-Type Cellulases Produced by Aspergillus niger, Trichoderma reesei, and Irpex lacteus on (1→3),(1→4)-β-D-Glucans and Xyloglucan
- セルラーゼおよびその関連酵素によるセルロース分解に関する新しい知見
- Inactivation of Hexokinase and Glucose-6-phosphate Dehydrogenase during Immobilization by Polyacrylamid Gel Entrapping
- Hydrogen evolution by a thermophilic blue-green alga Mastigocladus laminosus
- Mixed Culture of Mycotorula japonia and Pseudomonas oleovorans on Two Hydrocarbons
- Construction and Analysis of a Bacterial Community Exhibiting Strong Chitinolytic Activity
- Structure and Characteristics of an Endo-β-1,4-glucanase, Isolated from Trametes hirsuta, with High Degradation to Crystalline Cellulose
- Enzymatic Production of Glucosylxylose Using a Cellobiose Phosphorylase-Yeast Combined System
- Gene Cloning of Cellobiohydrolase II from the White Rot Fungus Irpex lacteus MC-2 and Its Expression in Pichia pastoris
- Effect of gene disruptions of the TCA cycle on production of succinic acid in Saccharomyces cerevisiae
- COUMARIN UP-TAKE SYSTEM IN TOBACCO CULTURED CELL
- INDUCTION AND GENETIC ANALYSIS OF PHENYLALANINE AMMONIA-LYASE IN TOBACCO COULTURED CELL
- Purification and Characterization of Triacylglycerol Lipase from Aspergillus oryzae
- Cloning and Characterization of a Chitosanase Gene from the Plant Pathogenic Fungus Fusarium solani
- Isolation of genes coding for chitin-degrading enzymes in the novel chitinolytic bacterium, Chitiniphilus shinanonensis, and characterization of a gene coding for a family 19 chitinase(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Creation of Bacterial Cellulose-Fabric Complexed Material
- Comparative Study on the Ability to Produce Gentiobiose in Cellulose-producing Bacteria Asaia bogorensis and Gluconacetobacter xylinus
- Regulation of Adsorption Behavior of Carbohydrate-Binding Module Family 1 and Endo-β-1,4-Glucanase onto Crystalline Cellulose
- Chitiniphilus shinanonensis gen. nov., sp nov., a novel chitin-degrading bacterium belonging to Betaproteobacteria