Cloning and Characterization of Mycovirus Double-stranded RNA from the Plant Pathogenic Fungus, Fusarium solani f. sp. robiniae
スポンサーリンク
概要
- 論文の詳細を見る
A mycovirus (named FusoV) from the phytopathogenic fungus, Fusarium solani f. sp. robiniae SUF704, has two kinds of double-stranded (ds) RNA genomes, designated M1 and M2. The cDNAs were constructed from FusoV genomic dsRNAs. The sequences of M1 and M2 cDNAs comprised 1645 and 1445bp, respectively. Sequence analysis showed that each dsRNA had a single long open reading frame (ORF) on only one of the strands. M1 ORF encodes a 519-amino acid residue polypeptide with a predicted molecular mass of 60 kDa. RNA-dependent RNA polymerase-conserved motifs were identified in the predicted amino acid sequence, and the polymerase synthesized dsRNA in vitro. The M2 ORF encodes a polypeptide of 413 amino acid residues with a predicted molecular mass of 44 kDa. The predicted amino acid sequence contained the sequence corresponding to those found in the purified 44-kDa capsid protein of FusoV.
- 社団法人日本農芸化学会の論文
- 1996-05-23
著者
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Nogawa Masahiro
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Taguchi G
信州大・繊維・応生系
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Nogawa Masahiro
Department Of Bioengineering Nagaoka University Of Technology
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Shimosaka M
信州大・繊維・応生系
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Shimosaka Makoto
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Okazaki M
Dep. Of Applied Biology Fac. Of Textile Sci. And Technol. Shinshu Univ.
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Okazaki Mitsuo
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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城石 正弘
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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TAGUCHI Goro
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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KAGEYAMA Tsutomu
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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NAKATANI Aiko
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Nakatani Aiko
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Kageyama Tsutomu
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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