Preferential Synthesis of Extracellular Glucoamylase by Aspergillus niger : Stability of mRNA Associated with Membrane-boud Polysomes in Nongrowing Mycelia
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概要
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To test a working hypothesis that secretory enzymes such as glocoamylase are produced on membrane-bound polysomes associated with long-life mRNA, experiments were carried out and the following results were obtained. Most RNA in growing mycelia of Aspergillus niger could be extracted with acetate buffer containing Mg-acetate and KCl, but only 2/3 of RNA in nongrowing mycelia were extractable with the same buffer. THe residual RNA could be extracted with acetatebuffer containing Na-lauryl sulfate and deoxycholate. These two RNAs fractions are discussed and deduced to be cytoplasmic and membrane-bound RNAs, respectively. RNA extracted from growing mycelia showed four peaks of OD_<260> on a MAK column chromatogram. One of these peaks was determined to be the RNA incorporated very rapidly with ^<14>C-adenine, had a half-life of about 4 min and showed no hyperchromicity. These observations indicate that it might be mRNA. mRNA associated with membrane-bound polysomes was also isolated from non-growing mycelia by the same procedure as above; and then through pulse labeling tests with ^<14>C-guanine and chase experiments, the decay kinetics of the specific mRNA was found to be monomolecular having a decay constant of 0.035 hr-<-1> i.e. 20 hr in half-life. About 20% stimulation of ^<14>C-guanine incorporation into mRNA associated with the membrane-bound polysomes was observed on addition of maltose, an inducer of glucoamylase, compared to the non-addition control. The increments or decrements of the other RNA fractions observed were less than 5%. From the above results, it was suggested that extracellular glucoamylase of A. niger is synthesized on membrane-bound polysomes associated with highly stable mRNA.
- 公益社団法人日本生物工学会の論文
- 1978-08-25
著者
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Miura Toshiharu
Department Of Biochemical Engineering Faculty Of Pharmaceutical Sciences Osaka University
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Okazaki Mitsuo
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Okazaki Mitsuo
Department Of Biochemical Engineering Faculty Of Pharmaceutical Sciences Osaka University
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Terui Gyozo
Department Of Biochemical Engineering Faculty Of Pharmaceutical Sciences Osaka University:(present A
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