Enhancing Effect of Zinc on Hepatoprotectivity of Epigallocatechin Gallate in Isolated Rat Hepatocytes
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概要
- 論文の詳細を見る
The influence of metal ions (Fe^<+2>, Cu^<+2>, Zn^<+2>) on the hepatoprotective activity of epigallocatechin gallate (EGCG) against hepatotoxin-induced cell injury was investigated. Primary cultures of rat hepatocytes were treated with a well-known hepatotoxin, bromobenzene (BB), in the presence of EGCG only or EGCG plus each metal ion. After 24 h, 0.02 m_M EGCG did not show protective activity on the cultured hepatocytes. In contrast, the hepatocytes were protected against BB in the presence of 0.02 m_M EGCG and 0.02 m_M zinc. The addition of only zinc could not protect hepatocytes against BB. These results suggest that the formation of the zinc-EGCG complex is very important in the enhancement of the hepatoprotective activity of EGCG. The complexation of EGCG with zinc was confirmed by UV-VIS absorption spectroscopy.
- 社団法人日本薬学会の論文
- 2002-09-01
著者
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近藤 昌夫
Graduate School Of Pharmaceutical Sciences Osaka University:department Of Pharmaceutics And Biopharm
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Kawase Masaya
Graduate School Of Pharmaceutical Sciences Osaka Univ.
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近藤 昌夫
Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Yagi Kiyohito
Graduate School of Pharmaceutical Sciences, Osaka University
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Yagi K
Graduate School Of Pharmaceutical Sciences Osaka University
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Yagi Kiyohito
Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Yagi K
Osaka Univ. Osaka Jpn
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Kawase M
Nagahama Institute Of Bio-science And Technology
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Kawase M
Osaka Univ. Osaka Jpn
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MAEDA Hatsuo
Graduate School of Pharmaceutical Sciences, Osaka University
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OHMORI Hidenobu
Graduate School of Pharmaceutical Sciences, Osaka University
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Maeda Hatsuo
Graduate School Of Pharmaceutical Sciences Osaka University
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KAGAYA Noritaka
Graduate School of Pharmaceutical Sciences, Osaka University
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TAGAWA Yoh-ichi
Institute of Experimental Animals, Shinshu University School of Medicine
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NAGASHIMA Hitoshi
National Food Research Institute
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Kagaya Noritaka
Graduate School Of Pharmaceutical Sciences Osaka University
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Tagawa Yoh-ichi
Institute Of Experimental Animals Shinshu University School Of Medicine
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Yagi Kiyohito
Department Of Biocehmical Engineering Faculty Of Pharmaceutical Sciences Osaka University
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Kawase M
Research And Development Center Kobayashi Pharmaceutical Co. Ltd.
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Ohmori Hidenobu
Graduate School Of Pharmaceutical Sciences Osaka University
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