Improvement of Heat Stability of Yeast Lipase by Chemical Modification with a Heterobifunctional Photogenerated Reagent
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概要
- 論文の詳細を見る
In order to improve the heat stability of purified lipase OF 360 from Candida cylindracea, lysine residues of the lipase were modified with a heterobifunctional photogenerated reagent, N-hydroxysuccinimide ester of 4-azidobenzoic acid, and photolyzed subsequently in the presence of decanol, which was proved to be a suitable heat stabilizer. The modified lipase showed excellent heat stability, even in the absence of decanol in the reaction system, compared to the native lipase. The activities of the modified lipase were retained about 70% after heating at 50℃ for 15 min, while the native lipase lost about 80% of the original activities. The stabilization thus observed might be mainly caused by in situ formation of a cross-link between decanol and the lipase, and partly attributed to intra-molecular cross-linking in the lipase.
- 公益社団法人日本生物工学会の論文
- 1990-09-25
著者
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Kawase Masaya
Graduate School Of Pharmaceutical Sciences Osaka Univ.
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Kawase Masaya
Nagahama Institute of Bio-science and Technology
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Kawase M
Osaka Univ. Osaka Jpn
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TANAKA ATSUO
Laboratory of Applied Biological Chemistry, Department of Synthetic Chemistry and Biological Chemist
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Sonomoto Kenji
Lab. Of Functional Food Design Dep. Of Functional Metabolic Design Bio-architecture Center Kyushu Un
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Sonomoto Kenji
Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyoto University:(
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Sonomoto Kenji
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Sonomoto Kenji
Laboratory Of Microbial Technology Department Of Food Science And Technology Faculty Of Agriculture
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Sonomoto Kenji
Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci
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Sonomoto Kenji
Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot
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Kawase Masaya
Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot
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Kawase Masaya
Department Of Bioscience Nagahama Institute Of Bio-science And Technology
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Kawase Mitsuo
Ngk Insulators Ltd.
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Kawase M
Research And Development Center Kobayashi Pharmaceutical Co. Ltd.
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Sonomoto Kenji
Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculty Of Agriculture
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Tanaka A
Kao Corp. Wakayama Jpn
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Tanaka Atsuo
Laboratory Of Applied Biological Chemistry Department Of Synthetic Chemistry And Biological Chamistr
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