Synchronized Fresh Cell Bioreactor System for Continuous L-(+)-Lactic Acid Production Using Lactococcus lactis IO-1 in Hydrolysed Sago Starch

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概要

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• An efficient bioreactor, termed a `synchronized fresh cell bioreactor', was developed and consisted of a pH-dependent substrate feed system coupled with cross flow filtration and turbidity control. The effect of high dilution rate and high cell density coupled with high cell viability on the production of L-lactic acid in continuous culture by Lactococcus lactis IO-1 in enzyme-hydrolysed sago starch medium was investigated. For all changes in dilution rate, cells responded in a synchronized way to the addition of glucose by increasing the rate of biomass formation. Consequently, a glucose-free feed solution was required to maintain the cell concentration at a particular pre-set value. This set-up facilitated the maintenance of the cells in a permanent log phase. At a cell concentration of 15 gl^<-1> and a feed glucose concentration of 53 gl^<-1>, volumetric LA productivities of 8.2, 19.3 and 33.1 gl^<-1> h^<-1> were obtained at dilution rates of 0.21, 0.50 and 1.1 h^<-1>, respectively. The respective residual glucose concentrations in the spent medium were 1.90, 0.24 and 3.80 gl^<-1>. By increasing the cell density, the volumetric productivity increased proportionally. At high cell density, higher dilution rates resulted in lower lactate concentrations in the culture medium resulting in higher productivity. This reactor facilitated efficient operation with high cell viability by maintaining the cells in continuous growth phase for long-term fermentation. Therefore, the growth rate (μ) was calculated according to the Monod equation. Using this system, high specific productivities can be obtained which guarantees high commercial productivity at economical cost with only a small investment for setting up the sago industry.

• 社団法人日本生物工学会の論文
• 2002-03-25

著者

• Sonomoto Kenji

  Lab. Of Microbial Technol. Div. Of Microbial Sci. And Technol. Dep. Of Bioscience And Biotechnology

• Sonomoto K

  (present Address)department Of Biochemical Engineering And Science Faculty Of Computer Science And S

• Sonomoto Kenji

  Lab. Of Functional Food Design Dep. Of Functional Metabolic Design Bio-architecture Center Kyushu Un

• Sonomoto Kenji

  Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyoto University:(

• Sonomoto Kenji

  Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci

• Sonomoto Kenji

  Laboratory Of Microbial Technology Department Of Food Science And Technology Faculty Of Agriculture

• Sonomoto Kenji

  Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci

• Matsunaka Toshiyuki

  Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculty Of Agriculture

• ISHIZAKI Ayaaki

  Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bios

• Sonomoto Kenji

  Laboratory Of Industrial Biochemistry Department Of Industrial Chemistry Faculty Of Engineering Kyot

• Ishizaki Ayaaki

  Laboratory Of Microbial Technology Division Of Microbial Science And Technology Department Of Biosci

• Ishizaki Ayaaki

  Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculty Of Agriculture

• Ishizaki Ayaaki

  Department Of Food Science And Technology Faculty Of Agriculture Kyushu University

• Ishizaki Ayaaki

  Division Of Bioscience And Biotechnology Graduate School Of Bioresource And Bioenvironmental Science

• Ishizaki Ayaaki

  Laboratory Of Microbial Science And Technology Division Of Bioscience And Biotechnology Graduate Sch

• Ishizaki Ayaaki

  Laboratory Of Microbial Technology Department Of Food Science And Technology Faculty Of Agriculture

• Sonomoto Kenji

  Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculty Of Agriculture

• Kobayashi Genta

  Division of Microbial Technology, Ariake Sea Research Project, Saga University

• Ishizaki A

  Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculty Of Agriculture

• Kobayashi Genta

  Department Of Food Science And Technology Faculty Of Agriculture 46-09 Kyushu University

• Kobayashi Genta

  Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculaty Of Agricultur

• NOLASCO-HIPOLITO CIRILO

  Laboratory of Microbial Technology, Department of Bioscience and Biotechnology, Faculty of Agricultu

• シリロ ノラスコ

  九大院・農

• Nolasco‐hipolito C

  Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculty Of Agriculture

• Kobayashi G

  Division Of Microbial Technology Ariake Sea Research Project Saga University

• Kobayashi Genta

  Laboratory Of Microbial Engineering Department Of Food Science And Technology Faculty Of Agriculture

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