Accumulation of Polyphosphate and Substrate Gas Utilization Efficiency in PHB Accumulation Phase of Autotrophic Batch Culture of Alcaligenes eutrophus ATCC17697^T

概要

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The consumption of phosphate, formation of polyphosphate and the intracellular ATP level were compared in exponential phase and PHB-accumulating cells of Alcaligenes eutrophus. The phosphate consumption (mg per g dry cell weight) by PHB-accumulating cells was larger than that by exponentially growing cells. The phosphate consumed by the PHB accumulating cells was converted to polyphosphate, which was a storage substance for phosphorus. The intracellular ATP concentration in PHB-accumulating cells (expressed as μmol g^<-1> protein) was 1.5 times that in exponentially growing cells. The same proportional increase in H_2 oxidation was observed. From this result it was observed that the ATP pool size expressed in terms of ATP/g protein per mol H_2 product was the same in both culture phases.
公益社団法人日本生物工学会の論文
1992-11-25