Production of Poly-D-3-Hydroxybutyric Acid from Carbon Dioxide by a Two-Stage Culture Method Employing Alcaligenes eutrophus ATCC 1769^T
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概要
- 論文の詳細を見る
A two stage culture was examined as a new method for the cultivation of Alcaligenes eutrophus to produce poly-D-3-hydroxybutyric acid, P(3HB), from H_2,O_2 and CO_2 without danger of gas explosion. In this culture method, the microorganism was grown beforehand in an organic medium under heterotrophic conditions for exponential growth, and the cells were then cultivated for P(3HB) accumulation under autotrophic conditions in which the O_2 concentration in the substrage gas was below the explosion limit of 6.9%. Although the K_Lα of the fermentor used was not high (cα. 340h^<-1>), it has possible to obtain P(3HB) at a relatively high production rate (0.56-0.91g・dm^<-3>・h^<-1>) and concentration (15.23-23.91g・dm^<-3>) in the autotrophic stage, even under such a low O_2 concentration.
- 公益社団法人日本生物工学会の論文
- 1994-04-25
著者
-
Tanaka Kenji
Department Of Advanced Energy Engineering Science Interdisciplinary Graduate School Of Engineering S
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Ishizaki Ayaaki
Department Of Food Science And Technology Faculty Of Agriculture 46-09 Kyushu University
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