Sequence Analysis of Replication Origin of Plasmid pLS11 of Bacillus subtilis IFO 3022

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概要

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• The structure of a 1.6-kb SphI-HindIII DNA sequence necessary and sufficient for the replication of a 8.6-kb plasmid pLS11 of Bacillus subtilis IFO 3022,which is responsible for γ-polyglutamate production, has been characterized by using a trimethoprim (Tmp)-resistance gene derived form B. subtilis TTK24 chromosomal DNA as a selective marker. The 1.6-kb DNA sequence contains a rep gene encoding the protein (333 amino acids) essential for initiation of replication and a possible origin of replication. The predicted REP protein of pLS11 has an overall homology with the REP proteins of pUH1 (74.8% identity), pBAA1 (92.8%), and pFTB14 (78.7%) in Bacillus spp., pLP1 (42.1%) and pLAB1000 (36.3%) in Lactobacillus spp., and pUB110 (35.3%) and pC194 (37.4) in Staphylococcus aureus, but has not any similarity with the REP protein of the staphylococcal plasmid pT181.

• 社団法人日本農芸化学会の論文
• 1992-02-23

著者

• HARA Toshio

  Microbiological Genetics Division, Institute of Genetic Resource, Faculty of Agriculture, Kyushu Uni

• Saito Hiroyuki

  Department of Biophysical Chemistry, Kobe Pharmaceutical University

• Ogata Seiya

  Microbial Genetics Division, Institute of Genetic Resources,Kyushu University

• Hara T

  Microbial Genetics Division Institute Of Genetic Resources Faculty Of Agriculture Kyushu University

• Hara T

  Kyushu Univ. Fukuoka Jpn

• Ogata S

  Kyushu Univ. Fukuoka Jpn

• Ogata S

  Mie Univ. Mie Jpn

• Ogata S

  Microbial Genetics Division Institute Of Genetic Resources

• NAKAJIMA KAZUHIKO

  Department of Bacteriology, Hyogo College of Medicine

• Ishizaki Ayaaki

  Department Of Food Science And Technology Kyushu University

• Ishizaki Ayaaki

  Department Of Food Science And Technology Faculty Of Agriculture Kyushu University

• Ishizaki Ayaaki

  Division Of Bioscience And Biotechnology Graduate School Of Bioresource And Bioenvironmental Science

• Ishizaki Ayaaki

  Laboratory Of Microbial Technology Department Of Food Science And Technology Faculty Of Agriculture

• Hara T

  Kagoshima Univ. Kagoshima Jpn

• Hara Toshio

  Microbial Genetics Division Institute Of Genetic Resources Faculty Of Agriculture Kyushu University

• Ishizaki A

  Laboratory Of Microbial Technology Department Of Bioscience And Biotechnology Faculty Of Agriculture

• Hara T

  Laboratory Of Cellular Regulation Technology Graduate School Of Genetic Resources Technology Kyushu

• Ogata Seiya

  Microbial Genetic Division Institute Of Genetic Resources

• UEDA Seinosuke

  Department of Applied Microbial Technology, Kumamoto Institute of Technology

• Ueda Seinosuke

  Laboratory Of Microbial Technology Department Of Food Science & Technology Faculty Of Agricultur

• Ueda Seinosuke

  Department Of Applied Microbial Technology Faculty Of Engineering Kumamoto Institute Of Technology

• Nakamura Kenji

  Microbial Genetics Division Institute Of Genetic Resources Faculty Of Agriculture Kyushu University

• Nakajima Kazuhiko

  Department Of Bacteriology Hyogo College Of Medicine

• Nakajima K

  Microbial Genetics Division Institute Of Genetic Resources Faculty Of Agriculture Kyushu University

• Ishizaki Ayaaki

  Department Of Food Science And Technology Faculty Of Agriculture 46-09 Kyushu University

• Ueda Seinosuke

  Department Of Agricultural Chemistry Faculty Of Agriculture Hokkaido University

• Saito Hiroyuki

  Microbial Genetics Division Institute Of Genetic Resources Faculty Of Agriculture Kyushu University

• Saito Hiroyuki

  Department Of Anesthesiology Gunma University School Of Medicine

• Hara Toshio

  Microbial Genetic Division Institute Of Genetic Resources

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