Deletion of Single Amino Acid E235 Affects the Structure and Lipid Interaction of Human Apolipoprotein A-I C-Terminal Peptides

スポンサーリンク

概要

• 論文の詳細を見る

• The C-terminal domain of apolipoprotein (apo) A-I plays an important role in lipid binding. ApoA-I Nichinan, a naturally occurring human apoA-I variant with a deletion of E235 located in the C-terminus, is associated with low high-density lipoprotein (HDL) cholesterolemia. In the present study, a series of variant peptides corresponding to residues 220—241 of human apoA-I were examined to clarify the influences of E235 deletion (ΔE235) on the structure and lipid interaction of the C-terminal region. NMR studies demonstrated that in trifluoroethanol, apoA-I 220—241/ΔE235 peptide forms the α-helical structure similar to wild-type (WT) peptide. Circular dichroism measurements revealed that the interaction with phospholipid vesicles induced structural changes from random coil to α-helix both in apoA-I 220—241 WT and E235A, a variant with a negative charge ablation, peptides. These peptides also showed abilities to form HDL-like particles through microsolubilization of phospholipid vesicles, indicating that the negative charge ablation in E235 has no effect on the lipid interaction. By contrast, neither lipid binding-induced α-helix formation nor microsolubilization of vesicles were observed in apoA-I 220—241/ΔE235 and L230P, a helix-breaking variant, peptides. In addition, fluorescence measurements showed that tryptophan fluorescence intensity of apoA-I 220—241/F225W greatly increased upon lipid binding, while only a little increase was observed for the corresponding ΔE235 variant. Taken together, these results suggest that the deletion of E235 causes defective lipid binding of apoA-I Nichinan because of the impaired helix-forming ability of the C-terminal residues.

著者

• Tanaka Toshitaka

  Department of Biophysical Chemistry, Kobe Pharmaceutical University

• Tanaka Masafumi

  Department of Biophysical Chemistry, Kobe Pharmaceutical University

• Sugiura Makiko

  Analytical Laboratory, Kobe Pharmaceutical University

• Kawakami Toru

  Laboratory of Protein Organic Chemistry, Institute for Protein Research, Osaka University

• Aimoto Saburo

  Laboratory of Protein Organic Chemistry, Institute for Protein Research, Osaka University

• Saito Hiroyuki

  Department of Biophysical Chemistry, Kobe Pharmaceutical University

• Tanaka Toshitaka

  Department Of Biophysical Chemistry Kobe Pharmaceutical University

• Tanaka Masafumi

  Department Of Biophysical Chemistry Kobe Pharmaceutical University

• Tanaka Masafumi

  Kobe Pharmaceutical University

• Saito Hiroyuki

  Department Of Anesthesiology Gunma University School Of Medicine

関連論文

• Deletion of Single Amino Acid E235 Affects the Structure and Lipid Interaction of Human Apolipoprotein A-I C-Terminal Peptides
• Effective Cancer Targeting Using an Anti-tumor Tissue Vascular Endotheliumm specific Monoclonal Antibody (TES-23)
• IS4-01 Indications for thoracoscopic repair of congenital diaphragmatic hernia in neonates(Respiratory System/Emergency,Share Smiles with Sick Children, Dreams and Innovation for Pediatric Surgery)
• IS O-12 Prognostic factors in antenatally diagnosed congenital diaphragmatic hernia : liver herniation and pulmonary artery size(International Session 3 Chest and Others,Science and Art for Sick Children)
• Two Patients with Polymyositis or Dermatomyositis Complicated with Massive Pleural Effusion
• The N-Terminal Helical Segments Modulate Structure and Lipid Interaction of Human Apolipoprotein A-I
• Aneurysms in the Ascending Aorta Adherent to the Sternum After Cardiac Surgery
• Saccular Descending Thoracic Aortic Aneurysm With Dysphagia
• Psychosocial factors affecting psychosomatic symptoms in Japanese schoolchildren
• Finite Element Method for Nonlinear Wave Propagation
• Clinical significance of increased plasma leptin in patients with chronic heart failure
• A Nearly Perfect Total-Field/Scattered-Field Boundary for the One-Dimensional CIP Method
• Sequence Analysis of Replication Origin of Plasmid pLS11 of Bacillus subtilis IFO 3022
• Characterization of Lipid-Binding Domains in Human Serum Amyloid A Using Its Fragment Peptides
• Cloning and Characterizalion of the Genomic RNA Scquence of the Mumps Virus Strain Associated with a High Incidence of Aseptic Meningitis
• Isolation and Characterization of Mumps Virus Strains in a Mumps Outbreak with a High Incidence of Aseptic Meningitis
• Prospective comparison of delivery outcomes of vaginal births after cesarean section versus laparoscopic myomectomy
• Significance of pulmonary artery size and blood flow as a predictor of outcome in congenital diaphragmatic hernia
• Application of RT-PCR Designed from the Sequence of the Local SRSV Strain to the Screening in Viral Gastroenteritis Outbreaks
• Management of pulmonary hypertension in congenital diaphragmatic hernia : nitric oxide with prostaglandin-E1 versus nitric oxide alone
• Influences of a Single Amino Acid Deletion on Lipid Binding of Human Apolipoprotein A-I C-Terminal Peptides
• PD34 BIOTRANSFORMATION OF DEHYDROABIETIC ACID
• Evaluation of congenital left ventricular aneurysm in the fetus by velocity vector imaging
• The Lifetime of the Lowest 6^+ State in ^Fe and E2 Effective Charge
• Conformational Studies of Transmembrane Peptides in Sterol-sensing Domain of SCAP Molecule
• Attempt to quantify uterine involution using acoustic radiation force impulse before and after placental delivery
• Effect of amino acid residues on membrane curvature sensing of amphipathic helix in human apolipoprotein A-I
• Use of rocuronium-sugammadex, an alternative to succinylcholine, as a muscle relaxant during electroconvulsive therapy
• Detection of formation boundaries and permeable fractures based on frequency-domain Stoneley wave logs
• Physicochemical Mechanism for the Lipid Membrane Binding of Polyarginine : The Favorable Enthalpy Change with Structural Transition from Random Coil to α-Helix
• APPEARANCE OF RECTAL VARICES IN EXTRAHEPATIC PORTAL OBSTRUCTION AFTER TREATMENT FOR ESOPHAGO-GASTRIC VARICES : A CASE REPORT
• Design of Multi-layer Anode for Direct Methanol Fuel Cell

もっと見る 閉じる

スポンサーリンク